DNA replication lecture 15 and 16

DNA replication lecture 15 and 16 - DNA - Replication...

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Unformatted text preview: DNA - Replication Chapter 12 5 5 3 10.16 p.280 Information flow Mechanism of replication Possible models conservative semiconservative dispersive 12.1 p.17 Requirements of replication DNA template dNTPs (dATP, dGTP, dCTP, dTTP) Enzymes DNA polymerase Enzymes for unwinding DNA 5 5 3 Four Stages of Replication 1. Initiation 2. Unwinding 3. Priming & Elongation 4. Termination Initiation Origin of replication one in E.coli "replication Bubble" replication is bidirectional 12.11 p.325 i. dnaA binds to oriC, causing unwinding ii. Unwinding allows helicase and SSBPs to bind INITIATION dnaA UNWINDING Helicase unwinds, SSBPs keep DNA from re- annealing, 12.12 p.325 NOTE: helicase CANNOT initiate unwinding Helicase http://physics.illinois.edu/research/Highlights/UnwindingDNA.asp E. coli- hexamer Bacillus stearothermophilus-monomer HELICASE- unwinds DNA DNA Gyrase DNA gyrase reduces positive supercoil ahead of the fork to allow for continued unwinding 12.12 p.325 w.cid.csic.es/homes/roca/TOPOII.html Four Stages of Replication 1. Initiation 2. Unwinding 3. Priming & Elongation 4. Termination DNA Polymerase Three DNA polymerases isolated from E.coli by Kornberg 2 additional have been discovered Common characteristics: Catalyze phosphodiester bond between 3 OH and 5 PO 4 Direction is always 5' to 3 Needs a template strand Can only add to an existing 3' end cannot initiate de novo P P O O O O O O DNA Polymerase...
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DNA replication lecture 15 and 16 - DNA - Replication...

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