Expressed Sequence Tags

Expressed Sequence Tags - ESTs Expressed Sequence Tags By:...

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By: Tina Scott BSCI 222-7118 Uses: •Help find and describe genes •The information provided by ESTs is used to in genome mapping •An EST is used to find pieces of chromosomal DNA using base pair matching •Identify polymorphic gene markers, which are used in genetic and physical mapping •Find active genes in particular tissue or at a particular point in development Expressed Sequence Tags are produced by the reverse transcription of cellular RNA. This process produces fragments of DNA from the coding region of the gene. Sequenced parts of the DNA fragments allow identification. Interesting Facts: •Usually 200-500 nucleotides in length •An expressed gene is sequenced at one or both ends •Genome size and introns affect the effectiveness of ESTs •RNA is used to find protein-encoding genes of interest because only small percentage of DNA encodes the amino acids and less than 2% of the DNA encodes the amino acids of protein. http://www.biologycorner.com/resources/DNA-
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This document was uploaded on 11/04/2011 for the course BSCI 222 at Maryland.

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Expressed Sequence Tags - ESTs Expressed Sequence Tags By:...

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