BMB401 F00 TF - BMB 401 — Examination 5-00 FORM 2 During...

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Unformatted text preview: BMB 401 — Examination 5 December 12, 20-00 FORM 2 During this exam yen should not be in possession of a beeper, calculator or a cellular phone. Please place these instruments (after you turn it off”) in your book bag and place the bag against the wall at' the room. Please answer the multiple-Choice questions on the answer sheet and mark the f01‘1‘n a! of your test in the FM column on the sheet. THESE ARE STANDARD SINGLE ANSWER MULTIPLE CHOICE QUESTIONS. THE POSSIBLE ANSWERS ARE LABELED A, B, C, D AND E SELECT rHE SINGLE BEST ANSWER AND MARK THE APPROPRIATE CIRCLE ON THE SCANTRON ANSWER SHEET. (TOTAL 37 POINTS) 1. Glycerol—phosplmlc goes through the glycerol— phosphate Shuttle and its pmduct is then completely oxidized to carbon dioxide and water. What will be the not yield of ATP in the complete oxidation 01' egCCInl phosphate. excluding the yield of die glycerol- phosphate Shuttle? A. 2 B. 14 C. 17 D. 36 E. 38 2. Lipoic acid is involved as a coon-4.1m in all of the following reactions EXCEPT A. the reaction catalyzed by the pymvate dchydrogenase mold-enzyme complex B. the reaction catalyzed by the alpha-ketOglutarate dchydrogenase mu] tl—e112ytne cmnptcx C. the Oxidativc decarboxylation of branched-chain alpha-keto—acids derived from branched amino acids . transketolase there are no exceptions in A through D you 3. Pymvate can be converted into ALL the following products EXCEPT onein a single enzymatic step. The enzyme may be a single enzyme or a mold-enzyme complex and comma-bound intermediates can be ignored. Mark the exeption. A. oxaloacctate B. acetleoA C. acetaldehyde (in yeast) D. lactate E. glycerol 4. The following are pairs of membolic pathways. In all cases one patlmny oi" the pair occurs in a DIFFERENT compartment of the not] than the other EXCEPT: A. cholesterol synthesis———ketone body synthesis B. pentose phosrihatc pathway-“fatty acid synthesis C. pyrimidine biosynthcsisminitia] steps of the urea cycle D. protein synthesis——prenj.'lnt.ion (cg. farneajlation) of proteins E. mRNA synthesis-"InRNA translation 5. Many biochemical compounds have more than one function. Au exmnple is citrate. It. has all of the l'oIlowing functions EXCEFI‘: A. It is an intcnncdiatc in the synthesis of Billy acids from glumsc. B. It is an activator of the rate-limiting step of fatty acid Synthesis C. It is an intermediate in the tricarboxylic acid cycle. D. It is an inhibitor of the rate-limiting step of glycolysis. E. Its amide is an intcnncdiatc in the urea cycle 6. Many biochemical compounds have more than one function An example is G'I‘l’. It has all of the t‘ollmring functions EXCEPT: A. It is needed to produce UDPG for glycogen synthesis. B. It is synthesized in the tricarb0xylic acid cycle but it can also be made from GDP and ATP. C. It is used in the rate—limiting step of gluconeogenesis, the step analyzed by PEPCK. D. It is needed for loading attiinMCyl-IRNA onto the A~Site of the ribosome. E. It is needed for the translocation of a growing pcpt idc from the A-site to the P—site. 7. Cnrboxypcptjdascs are hydrolytic enzymes that A. carboxylate proteins with tlte help of the catheter biotin. B. hydrolyze proteins at their last amino acid. C. hydrolyze proteins at amino acids with carboxylic acids in their side chains (GLU and ASP). D. remove amino acids from the lRNA at the A site of the ribosome and transfer it to the growing peptide chain. E. hydrolyze carboxylic acids. 8. A non-competitive inhibitor A. Lowers the Km of an enzyme. B. Lowers the velocity of a reaction at all substrate concentrations C. Increases tlte Km of art enzyme D. Increases both the Km and the Vtunx. E. Decreases both the Km and the Vmax. 9. Which of the following statements is N01" true for post-translational modification of proteins by pltosphoryiation? A. Phosisliorylation is it om-‘nlcnt modification B. Phospharylmion can activate enzymes through cattl‘orinational changes. C. Pltosphoryiation is ineversible. D. Phosphorylation may result in the creation of binding sites for specific proteins. E. Kinasw may be located at the membrane. in the cytosol or in the nucleus. 10. Which of the following is a posttnmsdptiortttl modifi cation of mRNA? A. thphnrylation B. HydrnxylatiOn C. GlycoSylation D. Splicing E. Proteolytic cleavage 11. Which of the following statements about oncogenes is INCORRECT? A. They encode altered key regulatory proteins in signaling pathways. B. They include ever-expressed normal forms of regulatory proteins. B. They cannot be kimscs. D. They have normal onttntcrpans called (pmto)oncogmtes. E. The leztd to the escape of tumor cells from normal regulatory processos. 12. The side chain of the following amino acid is LEAST likely to be part of the active site of an enzyme that also requires a metal ion for its activity. A. iettc'tne B. cysteine C. aspsrtate D. glutamate E. histidinc 13. Which ONE of the following TCA cycle intermediates may be added or I'CIIIOVCd by other metabolic pathways? (four altenuttives only) A. isocjtrate B. OxaIDSHCCiImte C. cis-acottitate D. stlminyl CoA 14. In glycolysis ATP Synthesis is catalymcl by: (four altematives only) A. ltexokirtase B. pltosrtltnt‘ructokittasel C. g1ycerztldeltyde-3-phosphutc deltydrogenztse D. pyrumte killilSfi 15. Pltosriltofructokinase 1 can be inhibited by all of the 1' allowing EXCEPT": (font alternatives only) A. high Concentrations of ATP 3. citrate C. AMP D. iowpI-I 16. Glucmtcogcnic enzymes include all of following EXCEP'I‘: A. pyrtivate wrboxylztsc B. glucose—é—pltospltatztse C. pltospltoenolpyruvztte carbonykinase I). fructose 1,6 bispltosphatase E. phosphoglucomutztse 17.I'NADPIfl/1NM)P+} is maintained at a high level in cells primarily by: A. lactate deltydrogennse B. the combined actions of gluoose—fi—phosphate dehydrogettase and the next enzyme(s) in the sante pathway. the action of electron transport chain shuttle incchimisms Such as the on-glycempltosphate clcltydmgcnsse shuttle the combined actions nftrzutsketolase and traitsaldoiase U0 5““ l8. Vertebrate acetyl CoA carboxylasa: A. undergoes prototner-polyrncr interconversion during its physiological regulation B. is inhibited by an AMP-dependent protein kinase mediated phosphorylation C. requires biotin D. content in a cell responds to clumges in fat content in the diet E. all of the above are correct 19. A deficiency ofcamitinc might be expected to interfere with: A. beta-exidation of fatty acids B. kctono-body formation from acetyl CoA C. paimitalc s'nlhesis D. mobilization ofslorcd tiiacy] glycerol from adipose tissue E. uptake of fatty acids into cells from the blood 20. Which ONE of the following statements regarding lipoprotein lipase is CORRECT? (four alternatives only) A. is an intracellular enzyme B. fiznctions to mobilize stored triacylglyccrnl from adipose tissue C. is stimulated by an CAMP-dependent protein kirmse mediated phosphorylation D. is associated with capillaries 21. Which one of the following statements regarding prostaglrmdins is INCORRECT? A. they have a very short half—life B. they are synthesized only in the liver C. they generally act locally on or near Ihc Li55uc that produces them D. their common precursor is arachidonic acid E. their synthesis can be inhibited by a number 01" unrelated compounds including cortisol and aspirin 12. Which ONE of the following statements best describes the fine of propionyl CoA in manunaiian Systems? A. it is Converted to aoety] CoA B. it condenses with acetyl CoA to form a S-carbon preCursor ora TCA Cycle intermediate C. it is metabolized via :1 reaction sequence that involves biotin and Vitamin B I 2 D. it is oxidized to malonic acid + CoA E. its methyl group is transferred to S— adenosylhomocysteine to form S- adenosylmethionine 23. which ONE of the following statements abant the synthesis of catbamoylphosphatc Synthetase l is INCORRECT? A. the enzyme catalyzes die mte~dctcnnining step in the urea cycle B. the reaction is allostericaily activaled by N- aoetngtutanmto C. the reaction requires two high energy phosphates for each molecule of carbamoylphosphate synthesized D. the enzyme incorporates 00; into carbamoylphoSphate E. the reaction is reversible 24. Which ONE of the following slaloments is CORRECT? A. chymotrypsin acts in the stomach on peptide bonds formed by the amino acid glycine B. nypsin is an exopeptidase Ihal liberates Free amino acids C. pepsinogen is aclivatcd by enteropetidase D. pepsin is an endopeptidaso secreted by the pancreas E. trypsin can act as an aclivator for all zymogens of pancreatic proteases 25. Citrate is important in metabolism and metabolic control because: . it is part of transport system for acctyl groups it is an intermedimc in the TCA cycle it stimulates fatty acid Synthesis it inhibits phosphofmciokinas‘: 1 all of the above are correct muon> 26. Which ONE of the following organs and tissues exports iduoose? A. adipose tissue B. skeletal muscle C. liver D. brain E. heart 27. The God cycle involves exchange of metabolites between (change one); A. liver and brain B. liver and muscle C. heart and adipose tissue D. heart and liver E. heart and muscle 28. All of the following stalcrncnts about amino acid Arc correct EXCEPT: A. ainmoacyl-tmnsfcr RNA {lRNA} sjt-nthetasc tran sfcr the amino acids to the adenine ( A) on me 3’ end of tRNA via an aminoacyladenosine monophosplmtc (AMP) intcnnccliate. B. the aminoacyl-LRNA synthetase is specific for a given amino acid. C. there may be more man one kind of tRNA for a given amino acid. D. the activation step requires the hydrolysis of the terminal phosphate group of guanosine triphoSphatc (GIT). E. once an amino acid is attached to a given IRNA, the specificity for recognition of the appropriate codon on messenger RNA (mRNA) is due entirely to the rRNA, and not at all to the amino acid. 29. All of the following factors are required to initiate translation of messenger RNA (mRNA) in prokaryotes EXCEPT: A. formylmenthiomne—transfer RNA; (tRNAr) B. 303 ribosomal subunits C. guanosinc triphnsphzitc (61'?) D. mRNA E. a 7 methyl-gmnosinc a1tnchcd"'backward“ to the 5‘ cud of the mRNA 30. Which of the following Sequences is most likely to be a restriction enzyme recognition silt: in doLtblc helicai DNA? A. CGGC B. CGC C. GTAATG D. OTC-GAG E. Then: is insufficient information 3 1. Restriction enzymes are best described by which one of the Following statements? A. They ligalc termini of necornbinant DNA molecules B. They confera selective advantage 0n invading bacteriophage C. They are enzymes that recognize and mcthylatc specific DNA sequences D. They make sequence—specific cuts in both strands of duplex DNA E. They cleave 5’ tenninal nucleotides from duplcx DNA molecules 32. An amino acid that has a polar side chain that docs nor ionize in solution is: A. Lysine B. bencine C. Alanine D. Aspanlgine E. Histidine. 33. The best definition of an endonnclease is an enzyme that hydrolyzes: A. a nucleotide from only the 3‘ end of an oligonucleotide. B. a nuclooLidc from either terminal of an oligonucleotidc. C. a phasphodiester bond located in the interior of a polynncleotide. D. a bond only in a specific sequencc of nucleotides. E. only single stranded DNA. 34. A paiindrome is a sequence of nucleotides in DNA that: . is highly reiterated. is part of the introns of eukaryotic genes. is a structural gene. . has local symmetryr and may serve as a recognition site for various proteins. has the infonnation necessary to confer antibiotic resistance in bacteria. m com» 35. Prokaryotic ribosomes: . are ribonucleoprotein complexes. Possess a pcptidyl transfcnise catalytic componenl in their smaller (30$) subunit. Act as primers for RNA Synthesis. Bind to the mRNA precm‘sor to recognize the 5 ‘— splice site for intron removal. E. Function in the postLranscriptional processing of RNA. an n» 36. The components of the polymerase chain reaction are a DNA template, deoayribonucleoside t'riphosphatcs, and DNA polymerase frOm Thai-mus aquaticus, an organism that rior‘rnall}r lives at high temperatures. These components are mixccl and placed into a thermal cycler, which will cycle repeatedly atthe temperature of 55°C. 72°C, and 92°C for various periods of incubation. What is the major Function ofthc 92°C temperature? It is the optimal temperature for DNA polymerization. To increase thr: Specificity of the binding of the pn' mcrs. To <lcru1turc the DNA strands. To drive the polymerase reaction to completion. E. To re—zuuteal newl)r synthesized DNA strands. F30??? 37. The carbohydrate chains in a glycoprotcin A. can increasa the stability B. in some cases are required for activity C. may have a role in targeting (localization) D. can be important for its interactions with cells and other proteins E. all of the above are correct in different glycoproteins. 38. In the Linewcaver Burke plot (l/v vs. i/[SD A. the slope is equal to l/Vm B. the intercept on the vertical axis is Vnt C. the intercept on the horizontal axis is -l/Km D. slope divided by intercept on y axis is Km E. slope divided by intercept on horizontal aids is -Vm 39. The Km for it reaction is always equal to A. The concentration of S required to give maximum velocity. 3. The equilibrium dissociation constant ofthc ES complex C. The substrate concentration that allows the enzyme to function at. half its maximum velocityr D. The number of molecules of product producud by each enzyme molecule per unit time E. The rate of association of E and S. 40. An ems-rue: A. increases the amount of product formed at the equilibrium of the reaction. B. decreases the equilibrium constant (Keq) of the reaction. C. decreases the standard free energy change 0]" the reaction. D. slows the attainment of equilibrium in the reaction. E. decreases the energy of activation for the reaction. 41. Which of the following statements about protein stnrcture is CORRECT? A. Quateman' structure requires cm'aleut interactions far stabilization. B. Beta-bends in peptide chains are oflcn caused by the presence of prolino. C. The alpha—helix can be composed of more than one polypepti de chain. D. internal ionic interactions do not stabilize tertiary structures of globular proteins. E. Hydrophobic side chains of amino acids must be symmetrically arranged around the outside of alpha~ helix secondary structures. 42. in solution at pH 7.1, tyrosine would possess: A. two negative charges and one positive charge. B. two positive charges and (inc negative charge. C. no net charge. D. a positive charge on its amide group. E. no ionic charges. 43. Which of die following is a imino acid? A. Leucine B. Glutamate C. Proline D. Lysine E. Glutzuninc 44. All of the following enzymes EXCEPT which One require prctcolytic cleavage as a requirement for activation? A. Collagen B. Pepsinogcn C. Neumpcptides D. Hemoglobin E. Polypeptide growth factors. Match the numbered enzymes in questions 45-48 with one of die descriptions of its action in the five lettered options given below. A. It alters the superc-oil structure of DNA. B. It hydrolyzes the RNA primers of replication. C. it catalyzes the formation of phosphodiester bonds between two DNA chains. D. It tmwinds the DNA preceding replication forks. E. It is the primary polymerization enzyme in replication. 45. Helicase 46. DNA Folyinerse ] (pol D 47. Gyrasc, a topoisomcmse 48. Li gase Match the numbered statement or phrase in questions 49- 51 with one of the FIVE lettered options given below. Below are five of the 6 classes ofcnzymes. Use these to match with the next diree numbered questions (9—11). A. Oxidomdnctases B. Transferascs C. Hydrolascs D. Lye-$05 E. Isomerascs 49. To which class belongs the enzyme that reversibly converts pyruvate to lactate? 50. To which cL'tss belongs the enzyme that synthesizes the peptide bond on the ribosome'! 5] . To which class belongs the enzymes that attach carbohydrates or lipids eminently to proteins? ...
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