BMB401 F09 T2

BMB401 F09 T2 - Cam I, (Name) BMB 401/506-Fall 2008 Exam 2...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 2
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 4
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Cam I, (Name) BMB 401/506-Fall 2008 Exam 2 Form 2 October 13, 2009 During this exam you should not be in possession of any electronic equipment (beeper, calculator, cellular phone, earphone, etc.), books and papers, other than the test. Please place all these materials in your book bag (after you turn them off! I) and place the bag against the wall of the room. THESE ARE STANDARD ANSWER MULTIPLE CHOICE QUESTIONS. THE POSSIBLE ANSWERS ARE LABELED A, B, C, D AND E. SELECT THE SINGLE BEST ANSWER AND MARK THE APPROPRIATE CIRCLE ON THE SCANTRON ANSWER SHEET. Bubble in your name and C# and write your seat number. There are 40 questions. You will receive full credit for 38 correct answers. 1. Which of the following statement(s) about glucokinase is(are) 1N CORRECT? A. It is present in neural tissue B. Its KID for glucose is much higher than that of hexokinase C. It is inhibited by glucose—fi—phosphate D. A and B _ 2. The product of ONE of the glycolytic enzymes is also formed by the combined actions of two enzymes of the gluconeogenic pathway. Name the glycolytic enzyme? A. Hexokinasefglucokinase B. Phos hofructokinase I * D. Glyceraldehyde-3-phosphate dehydrogenase E. Enolase 3. If NAD” were not to be regenerated under aerobic or anaerobic conditions, which enzymatic step of glycolysis will cease to function? A. TriosephOSphate isomcrase B. Phosphoglucose isomerase . "‘T'T -p 05 ae e yrogenas D. Phospho g ycerate mutase E. None of the above 4. Complete oxidation of a mole of glucose to C02 and H20 produces far less ATP than does the complete oxidation of a mole of palmitic acid. Why? A. Palmitic acid is insoluble in water while glucose dissolves readily. B. Palmitic acid is mostly hydrophobic while glucose is hydrophilic in nature. - ;1 O - - - have neither received nor given help and observed the Honor Code 5. During fasting, blood glucose homeostasis is maintained because: A. Phosphorylation of phosphorylase enhances liver glycogen breakdown B. Phosphorylation of phosphorylase enhances muscle glycogen breakdown C. Increase in the activity of phosphoenolpyruvate carboxylcinase (PEPCK) D. A - I B 6. Increased glucagon levels enhance: A. Glycolysis B. GlucOneogenesis- C. Glycogen breakdown D. i DB 7. Insulin rec'eptor: A. Dimerizes following insulin binding B. Has intrinsic tyrosine kinase activity C. Insulin binding causes autophosphorylation of its D. MShlrn‘Wmv' él hid) 0"! tyrosine residues Phosphorylation of tyrosine residues results in the hos - horylation of insulin receptor substrates (IRS) ‘ 8. Glucose synthesis from lactate requires: A. NADH B. ATP C. G'I‘P D. 9. One of the statements regarding glucose—fi-phosphatase is INCORRECT. Which ONE? ’6'. Is present in hepatocytes (liver cells) fl Is bound to the luminal side of the endoplasmic reticulum I). Is a protein phosphatase 10. Protein kinase C has all of the following characteristics EX . A. I Is activate y cclic u -'- equirs a or activity ' 49 Requires diacylglycerol for activity D. Catalyzesphosphorylation of serinev'threonine residues of its substrates 11. Which ONE of the following adducts resulting from glucose- 1-phosphate and a nucleoside triphosphate (in a reaction catalyzed by pyrophosphorylase) provides all the glucosyl residues for the synthesis of glycogen? A. ADP-glucose B. GDP-glucose C. CDP- glucose E. None of the above BMB 401 @2009 Department of Biochemistry & Molecular Biology, University of Miami, reproduction prohibited. Page 1 0f 4 I, (Name) 12. The process by which GTP is formed from GDP in the mitochondria is referred to as: A. Auto-phosphorylation B. Oxidative phosphorylatiOn swarmi- D. The electron-transport chain E. Trans-phosphorylation 13. The maximum net yield, in moles, of ATP from the complete oxidation of One mole of glycerol is: A. 1 B. 3 C. 15 $9 E. 36 14. NADPH that is generated by the oxidative branch of the pentose phosphate pathway is used for all EXCEPT: A. Reduction of oxidized cysteine residues of proteins B. Reduction of oxygen radicals via glutathione C. {-u ' w. l esisoflipids 4 I11. fATPviatheeecontransr . E. There is no exception 15. Malate: XE) 'OOC—CHZ-CHZ—COO' B. 'OOC~CH2—CHO—COO' C. 'OOC-CH(OH)—CH2~COO‘ D. 'OOC—CO-CH2-COO' " -'- E. 'OOC-CH=CH—COO' 16. Ribulose: A. HOCfi-IZ)-HCOH—HCOH-HCO B. HOC(H2)—HCOH—HCOH—CO-H2COH c. HOC(H2)—HCOH—HCOH-HCOH—H2COH (Em0C(H2)—HCOH-Hc0H-HCOH—Hco E. HOC(H2)-HCOH-HCOH—HCOH-HCOH—HCO 1?. Oxaloacetate A. Is a four carbon dicarboxylic acid B. Is formed from the oxidation of malic acid C. is formed by the carboxylation of pyruvate D. Is essential for the entry of acetyl CoA into the TCA le _ E. Alloftheao 13. The phosphoinositol portion of the phosphatidylinositol molecule is called: _ I . Am hipathic moiet B. Hydrophobic mo - i C. Hydrophilic moiety D. None of the above “fill—'- 19. How many tricarboxylic acids are found in the TCA cycle? @ Do have neither received nor given help and observed the Honor Code 20. Which of the following substances is NOT a membrane lipid? W B. Phosphoglycerides .=D.-meervtams E . H M] 21 . Which of the following statements about CAMP and its role in hormone action are CORRECT? A. Nearly all effects of CAMP in eukaryotes are exerted through the activation of protein kinase A (PKA) B. It binds the regulatory subunits of PKA and activates the enzyme by releasing the catalytic subunits C. It binds the catalytic subunits of PKA and activates the enz me allosterically 4mg:- 22. Which of the following are CORRECT statements about G~proteins that function in CAMP-mediated hormonal systems? These G-proteins A. bind hormones B. are heterotrimers C. When the GDP-bound form binds to a hormone- receptor complex, GTP replaces GDP INCORRECT? A. It is the major electron acceptor used in fuel __ met olism t contains a nicotinamide ring that accepts a hydride ion during reduction D. it contains an adenine nucleotide as a part of its structure 24. Which ONE of the following is a high-energy compound? A. Glycerol-B-phosphate B. Glucose-fi-phosphate C. Fructose 1, 6 bisphosphate W E. Glucose-l-phosphate 25. Which ONE of the following water-soluble vitamins forms part of the structure of CoA? A. Niacin B. Biotin C. Riboflavin Jim-mm E. Vitamin B12 BMB 40] @2009 Department of Biochemistry & Molecular Biology, University of Miami. reproduction prohibited. Page 2 of 4 i, (Name) 26. Which ONE of the following statements regarding the TCA cycle is INCORRECT? X Oxidizes acetyl CoA derived from fatty acids and other compounds . Provides precursors for the biosynthesis of aSpartic and glutamic acids 1 Produces one mole of GTP from GDP X Synthesis of oxaloacetate, required to begin the cycle occurs by an anaplerotic reaction a High energy electrons produced during the cycle are captured by NADP+ and FAD 27. Which ONE of the following vitamins is NOT involved in the reaction mediated by the pyruvate dehydrogenase complex? : _ ‘1cotinamide C. Pantothenic acid D. Riboflavin E. Thiamine 28. Select die correct pair of nucleoside triphosphate involved in the synthesis of glycogen from glucose? A. CTP+UTP B. GTP+CTP C. TTP+UTP E. + P 29. Which ONE of the following cellular compartments (organelles) is NOT involved in the synthesis of glucose from lactate? _ A. Mitochondrion B. Endoplasmic retiCulum/ C. C tosol 30. Regulation of metabolic pathways is accomplished in many ways. Which of the following is NOT one of the major regulatory strategies? g: Allosteric control mechanisms Location of competing pathways (synthesis and degradation) in the same organelle \C. Feedback inhibition \D. Control of l_' veI of enzyme - "u - anslational mor --;' _ - r nerally extracted from ingested foodstuff by: .: drol ss rea I _ I B. Condensation and cleavage reactions C. Oxidation and reduction reactions D. Acidfbase reactions E. None of the above provides a suitable explanation have neither received nor given help and observed the Honor Code 32. How many mechanistically distinct substrate-level phosphorylation reactions occur in glycolysis? A.0 B.l_ ((2:22) D3 54 33. The glycolytic enzyme influenced as a result of glucagon action (reversible phOSphorylation) is: A. Glucoldnase B. Pyruvate kinase C. Triosephosphate isomerase D. Aldolase _— Ph'sp og yce a u a. 34. How many moles of NADH are produced during the conversion of glucose to pyruvate? A.0 HI 41223 13.3 E4 35. Which enzymes of the TCA cycle catalyze oxidative decarboxylation reactions? A. Aconitase and succinate deh dro ena - B" soc: ate e ydrogenase and a-ketocrlu _. ‘ - utarate dehydrogenase complex and succinate thiokinase D. Fumarase and succinate dehydrogenase E. Malate and succinate dehydrogenaSe 36. The only membrane bound enzyme in the TCA cycle is: A. Malate dehydrogenase B. a-Ketoglutarate dehydrogenase complex D. Citrate syn ase E. Fumarase 37. A TCA cycle intermediate NOT used in biosynthetic reactions: )6 Oxaloacetate C. o-Ketoglutarate HMCCiHyI'COA JW 38. Which of the following represents the primary function of the pentose phosphate pathway? A. To synthesize pentose phosphates and NAD+ B. To produce NADH C. To convert pentose phosphates to metabolic ' ‘ for oxidative phos ho lation hyaluronic acid BMB 401 @2009 Department of Biochemistry & Molecular Biology, University of Miami. reproduction prohibited. Page 3 of 4 I, (Name) have neither received nor given help and observed the Honor Code 39. Starting with fructose-fi-phosphate and proceeding to pyruvate. what is the net yield of ATP molecules? r l. 41. I wrote my name, as on fire class roll. my C-number and seat number in the appropriate places on the scantron sheet. mm A. 1 .4 ,- - " B. No (one point penalty for each omission! C' 3 42. [. properly. bubbled in my name. as on the class roll and my C—nnmher D. 4 in the appropriate places en die scantron sheet. E. 5 AI ' T'fesifit—fib B. No (one point penalty for each omission) 40. Which ONE of the following statements about pyruvate carboxylase is CORRECT? : Requires NADH for activity /B./Is present in the cytosolic comparnnent Wtyl CoA /Bf“ Requires ATP for catalysis E. Requires thiamine pyrophosphate for activity 43. I, properly, bubbled in the form number of this test in are appropriate place on the scantron sheet. A: “Yes h B. No (Three (3) point penalty for form omission or error!!!) The form number of this test is 2 The genetic code First Base J, Second base in mRNA —> U C A G U UUU Phe (F) UCU Ser (8) UAU Tyr (Y) UGU Cys (C) UUC Phe (F) UCC Ser (8) UAC Tyr (Y) UGC Cys (C) UUA Leu (L) UCA Set (8) UAA Stop UGA Stop!H UUG Leu (L) UCG Ser (8) UAG Stop UGG Trp (W) C CUU Leu (L) CCU Pro (P) - CAU Hie (H) CGU Arg (R ) CUC Leu (L) 000 Pro (P) CAC His (H) CGC Arg (R ) CUA Leu (L) CCA Pro (P) CAA Gln (Q) CGA Arg (R ) CUG Leu (L) COG Pro (P) CAG GM (0) CGG Arg (R ) A AUU He (1) ACU Thr (T) AAU Asn (N) AGU Ser (8) AUG lle (I) ACC Thr (T) AAC Asn (N) AGC Ser (8) AUA lle (I) ACA Thr (T) AAA Lys (K) AGA Arg (R ) AUG Met (M) ACG Thr (T) AAG Lys (K) AGG Arg (Fl) G GUU Val (V) GCU Ala (A) GAU Asp (D) GGU Gly (G) GUC Val (V) GCC Ala (A) GAC Asp (o) eec Gly (e) GUA Val (V) GCA Ala (A) (3AA Glu (E) GGA Gly (G) GUG Val (V) GCG Ala (A) GAG Glu (E) GGG Gly (G) ** = selenocysteine in defined context BMB 401 @2009 Department of Biochemistry & Molecular Biology, University of Miami, reproduction prohibited. Page 4 0f 4 ...
View Full Document

Page1 / 4

BMB401 F09 T2 - Cam I, (Name) BMB 401/506-Fall 2008 Exam 2...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online