Topic 13, replication I.ppt.edu

Topic 13, - Topic 13 DNA replication part 1 Learning objectivesTopics 13 and 14 Be able to define the following terms DNA polymerase I and III

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Topic 13: DNA replication part 1
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Learning objectives—Topics 13 and 14 Be able to define the following terms: DNA polymerase I and III, helicase, primase, Rnase H, ligase, pulse-chase experiment, semi-conservative & semi-discontinuous DNA replication, dimer, leading strand, lagging strand, reverse transcriptase, telomerase, topoisomerase Be able to explain the goals of a cell when performing DNA replication Be able to explain why synthesis of the 2 different DNA strands must be fundamentally different from each other Be able to diagram how DNA replication takes place, with emphasis on lagging strand synthesis Be able to explain the contributions of the various enzymes & proteins to DNA replication
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Focus on DNA replication in bacteria Why? Best studied system Very similar in eukaryotes, but names of proteins can change
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What are the goals of DNA replication? Make complete new copies of genetic material in preparation for cell division so that each daughter cell has same genes/traits as parent cells Maintain genetic information with accuracy What happens if 1 incorrect nucleotide is inserted?
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What about pathogenic viruses and bacteria? Do they want to maintain genetic information that is 100% the same as the parent cell? Why or why not?
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New DNA, already synthesized Next building block for polymer
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Group discussion: 1.Which model turned out to be correct? 2.Why does it matter which model is correct? --Would one model be more likely to result in the perpetuation of errors?
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which is essential and almost always going on in at least some cells in a population? Such as DNA replication?
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This note was uploaded on 11/04/2011 for the course MMBIO 240 taught by Professor Bridgewater during the Fall '09 term at BYU.

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Topic 13, - Topic 13 DNA replication part 1 Learning objectivesTopics 13 and 14 Be able to define the following terms DNA polymerase I and III

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