{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Topic 17, homologous recombination.ppt.edu

Topic 17, homologous recombination.ppt.edu - Topic 17...

Info icon This preview shows pages 1–10. Sign up to view the full content.

View Full Document Right Arrow Icon
Topic 17: Homologous Recombination
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Learning objectives Be able to define the following terms: homologous sequences, DNA recombination, crossing over, genetic linkage, Holliday junction, DNA resolution Be able to list the molecular events required for homologous recombination (slide 11) Be able to list 3 ways in which DNA recombination can play a role in repairing DNA damage Be able to draw a diagram of a Holliday junction, and explain the 2 possible ways to resolve the junction Be able to explain the practical application of homologous recombination to manipulate complex genomes
Image of page 2
Terms to understand Homologous DNA sequences: 2 DNA molecules with very similar DNA sequences in the same order Need not be 100% match, but should be pretty close to 100% for efficient recombination rates DNA recombination: process of introducing new DNA sequences into existing DNA (often includes swapping sequences between 2 strands of dsDNA)
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Homologous recombination: the big picture Required for: Repairing dsDNA breaks Correct chromosome segregation in meiosis Also contributes to: Genetic diversity Process can be manipulated to generate recombinant organisms, especially useful for complex (large) genomes: can’t just cut and paste when millions of the same palindrome exist in the genome
Image of page 4
Terms to understand Crossing over: homologous (very similar) DNA sequences from different chromosomes line up next to each other and switch places Genetic linkage: a method to determine how close 2 genes are to each other based upon the frequency of crossing over together vs. crossing over separately
Image of page 5

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
How does this happen? Homologous recombination: the big picture
Image of page 6
Unlinked genes: they segregate independently Linked genes: they nearly always segregate together. They can segregate independently if crossing over takes place between them, but if they are very close to one another then likelihood of crossing over independently is very low
Image of page 7

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Example of how linked genes can segregate independently: the closer they are to each other, the less likely this will occur
Image of page 8
Visualization of a crossing over event
Image of page 9

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 10
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern