Topic 24, RNA pol II core basal transcription.ppt.edu

Topic 24, RNA pol II core basal transcription.ppt.edu -...

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Topic 24: RNA polymerase II core basal transcription (eukaryotes)
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Learning objectives Be able to define the following terms: core promoter, TATA box, initiator sequence, S1 nuclease mapping, run-off transcription, primer extension, RNA pol II basal transcription complex, general transcription factors Be able to draw/label the names and locations of the core promoter area for eukaryotic RNA pol II (only DNA sequence to know is TATA; slide 3) Be able to describe & differentiate methods used to map the initiation site of transcription (slides 10-13) Be able to describe the functions of each of the RNAP II transcription factors in the basal complex and the order in which they assemble to form the basal complex Be able to describe the major features of how the complex proceeds from the initiation phase to the elongation phase of transcription
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Figure 15.17: Core promoter motifs. -40 to +40 portion of the template is considered the “core promoter” area for RNA pol II *TATA box is equivalent to the -10 promoter in prokaryotes; farther away from transcriptional start site in eukaryotes TFIIB Notation????
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“TATA or Pribnow box” Quick comparison of eukaryotic (top) and prokaryotic promoters
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There are 2 main types of promoters used by eukaryotic RNA pol II: One type has a TATA box Other has no TATA box, does have DPE
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TATA box sequence and location is highly conserved
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RNA Pol II promoter features TATA box : similar to the bacterial -10 sequence Consensus: TATAA At ~25-30bp upstream of start site Binds TBP (TATA-Binding Protein), which is a component of TF II D, the first transcription factor (TF) to bind DNA to initiate transcription (what does consensus mean?)
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RNA Pol II promoter features Initiator (InR) : the sequence surrounding and including the start site for transcription Consensus: Y Y A N A/T Y Y Pyrimidine-rich (Y = C or T) First base transcribed is often A
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RNA Pol II promoter features Downstream promoter element (DPE) : Not present in every promoter Often present in TATA-box-less promoters At around +28 to +32
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Topic 24, RNA pol II core basal transcription.ppt.edu -...

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