Topic 25, promoters and enhancers.ppt.edu

Topic 25, promoters and enhancers.ppt.edu - Topic 25:...

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Topic 25: Promoters and enhancers
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Last hour: The RNA Pol II basal transcription complex— the basic factors essential for transcription This hour: Regulating eukaryotic transcription—how DNA sequences and activator proteins recruit the basal transcription complex to specific DNA sites; also how to keep transcription going once it has already started
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Learning objectives Be able to define the following terms: DNA binding domain, enhancer (aka, upstream activator sequence or UAS), activating domain, regulatory promoter, activator, co-activator, Mediator complex Be able to differentiate between the 3 main control regions for transcription: core promoters, regulatory promoters, and enhancers (function, general location) Be able to describe the main attributes of transcriptional activators (slide 18) Be able to describe the necessary of events for effective eukaryotic transcription initiation (summary on slide 23) Be able to differentiate between 4 methods to map out DNA sequences involved in transcription initiation (slides 25-33) Be able to list the 4 common DNA-binding motifs (slide 36) and be able to explain why there are a limited # of such motifs
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Now we see that there is more to it than just the basal transcription complex….(topic 24) Topic 24: basal complex assembles here and starts transcribing here Topic 25: formation of basal transcription complex is inefficient unless other upstream regulatory regions are involved
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Core Promoters Located in the direct vicinity of the transcriptional start point Site where the RNA polymerase and associated transcription factors bind to initiate transcription Very similar for all genes transcribed by RNA pol II, because same basal transcription complex used for all genes transcribed by RNA pol II
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Regulatory Promoters Located further upstream (5’) than the core promoter Site where transcriptional activator proteins bind Activators bind to regulatory promoters and assist TFIID to recruit the rest of the basal transcriptional complex
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Enhancers are DNA sequences that: Can be far away from the promoter Can be upstream or downstream of the promoter, and even in an intron Still function when removed and reinserted in the opposite orientation Include multiple protein-binding sites/response elements
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Activator proteins interact with regulatory Basal transcription complex binds here Interact with RNAP via Mediator complex -200 to -50 -40 to +40
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Definition of 3 main types of Transcription Factors (TFs) • Basal factors (aka, general TFs): TF II D, TF II B, etc. Activators:
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This note was uploaded on 11/04/2011 for the course MMBIO 240 taught by Professor Bridgewater during the Fall '09 term at BYU.

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Topic 25, promoters and enhancers.ppt.edu - Topic 25:...

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