Topic 19, transcription in prokaryotes

Topic 19, transcription in prokaryotes - Topic 19:...

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Topic 19: Transcription in prokaryotes
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Focus of this unit (Topics 19-29): What components/proteins are necessary for transcription to take place? How does the cell regulate which genes are transcribed or not transcribed? **** What types of RNAs are produced and what are they used for? What is RNA maturation and why is it necessary? What differences exist between transcription in prokaryotes and eukaryotes? Be able to differentiate between DNA replication and RNA transcription!!!
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Learning objectives Be able to define the following terms: RNA polymerase, core enzyme, holoenzyme, sigma factor, transcription factor, terminator, ‘upstream’, ‘downstream’, promoter Be able to predict/draw the RNA sequence from a labeled dsDNA template sequence Be able to describe the 3 phases of RNA transcription (slide 10) Learn the elements of a gene that control the starting and stopping of transcription (slide 11) Be able to differentiate between the mechanisms of RNA transcription and DNA replication (entire unit objective; see slide 14) Be able to explain what components are necessary for transcription to begin and end (DNA/gene components and polymerase components)
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Important terms defined Transcription: making RNA using a DNA template Gene expression: the process of using the information stored in DNA to DO SOMETHING (usually via protein production) RNA polymerase: enzyme that makes RNA from a DNA template Transcription factor: a molecule (usually a protein) that regulates whether or not transcription will take place or not take place Upstream and Downstream are terms to used to label gene sequences and are relative to base # +1 (see slide 11) Promoter: a region of DNA that is nearly always directly upstream of the start site of transcription. Transcription factors and RNA polymerase bind here to prepare to initiate transcription.
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What does the term gene expression mean? Why does gene expression need to be
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This note was uploaded on 11/04/2011 for the course MMBIO 240 taught by Professor Bridgewater during the Fall '09 term at BYU.

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Topic 19, transcription in prokaryotes - Topic 19:...

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