110 - Growth on lactose L L – – L – L – is...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
1) Very efficient transfer of markers carried on  FFFFF . These can be markers that were transferred very late  in the  HHHHH from which the  F was derived.  2) No transfer of chromosomal markers not on  FFFFF F s can be used to perform genetic tests of function because a cell containing a  FFFFF will be diploid for the  region of the chromosome carried on  FFFFF . This is known as a  mmmmm . For example, if we isolated a new  L mutation we could use an  F + to determine whether the  L – mutation is dominant or recessive. 
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Growth on lactose L + + L – – L – / + + ( L – is recessive) It is also possible to test for functional complementation of two linked mutations. Consider two mutations, A – and B–– , that are close together and have the same phenotype. We can introduce an FFFFF carrying A– into a strain with a B– mutation. If the merodiploid has a wild type phenotype then we know that the mutations complement and are therefore in different genes....
View Full Document

{[ snackBarMessage ]}

Ask a homework question - tutors are online