{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

By way of review - found in an almost unlimited number in...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
By way of review, let’s consider the general question of how genetic mapping studies can be used to locate a gene that has been identified by an allele with an interesting phenotype. For example, the CLOCK mutation in mouse was identified as a semi-dominant mutation that disrupts the normal circadian rhythm. This mutation was isolated after mutagenesis of animals with ethyl-nitorosourea and then screening their offspring for abnormal activity at a time when normal mice would be sleeping. Let’s assume that we have at our disposal a large number of genetic markers spaced evenly along all of the mouse chromosomes (later in the course we will discuss DNA-based markers which can be
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: found in an almost unlimited number in the mammalian genome) and that we have the capability to screen about 1000 mice for recombinants in a mapping experiment. The question of how precisely we can locate the CLOCK gene can be considered to be a question of the resolving power of a mapping experiment. Two points on a genetic map can only be resolved if a recombination event that separates them can be found. The smallest interval that can be resolved on average if 1000 progeny are screened is 1 recombinant in 1000 which corresponds to a map distance of ~ 0.1 cM on either side of the CLOCK mutation. Thus we would be able to map the CLOCK mutation to an interval of 0.2 cM....
View Full Document

{[ snackBarMessage ]}

Ask a homework question - tutors are online