Introduction of the Human β-globin gene with the sickle cell mutation ( β S H ) into the mouse genome: In the 1980’s and early 1990’s several groups tried to make a mouse with sickle cell disease by introducing the Human β-globin gene with the sickle mutation ( β S H ) , in the hope that if the protein was expressed at high levels it would precipitate Hb fibers that would cause sickling of RBCs, thus mimicking sickle cell disease . How does one make a transgenic mouse ? Mice are treated with a hormone to make them super-ovulate and then mated. Soon after mating, the fertilized eggs are retrieved from the uterus. Eggs that contain two pronuclei (one from the mother and one from the father) indicating that the embryo is still at the one-cell stage, are identified under
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