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Unformatted text preview: that contains histidine and also to a plate that lacks histidine. His insertion mutants would be identified as colonies that can not grow on the plates that lack histidine. Note that the same collection of random Tn5 insertions can be screened multiple times to find interesting mutations with different phenotypes. 3) Identify His TTTTT insertion mutants by replica plating to find colonies that specifically can not grow on plates that dont contain histidine....
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- Fall '10