R alleles usually cause the loss of something that is made in wild type

R alleles usually - in the same gene Say you isolate a new recessive histidine requiring mutation that we will call HisX – In principle this

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R alleles usually cause the loss of something that is made in wild type DDDDD alleles usually cause increased activity or new activity It turns out that the Cup r allele actually carries more copies of the gene for a copper binding protein and therefore increases the activity of the gene. Last lecture we defined the gene structurally as the DNA needed to encode a protein. We can now define a gene in a new way based on its function. Using the phenotypic difference between wild type and a recessive allele we can use a C CC C to determine whether two different recessive alleles are
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Unformatted text preview: in the same gene. Say you isolate a new recessive histidine requiring mutation that we will call HisX – . In principle, this mutation could be in His3 or it could be in any of the other genes in the histidine biosynthetic pathway. In order to distinguish these possibilities we need a test to determine whether HisX the same as His3. To carry out a complementation test, one simply constructs a diploid carrying both the His3 – and HisX – alleles. An easy way to do this would be to mate a MATα HisX – strain to a MAT aaaaa His3 – strain....
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This note was uploaded on 11/05/2011 for the course BIOLOGY MCB2010 taught by Professor Jessicadigirolamo during the Fall '10 term at Broward College.

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