True Breeding - Say we have isolated a new paralyzed mutant...

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True Breeding: homozygous for all genes Say we have a true breeding line of shibire flies these flies are paralyzed and have genotype shi– / shi– . First, we can test to see whether the shibire allele is dominant or recessive. shi– / shi– x (wild type)  shi+ / shi+ all are  shi– / shi+ (The offspring from a cross of two true breeding lines is known as the F1 or first filial generation). The F1 flies appear like wild type therefore  shi– is recessive (not expressed in heterozygote)
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Unformatted text preview: Say we have isolated a new paralyzed mutant that we call par . We start with a true breeding par– strain that we mate to wild type. We find that the mutation is not expressed in the F1 heterozygotes and therefore is recessive. To find out whether par– is the same as shi– we can do a complementation test since both mutations are recessive. For this test, we cross a true breeding par– strain to a true breeding shi– strain....
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