When λ λλ λ P P P

When λ λλ λ P P P

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
When λ λλ λ P P P – –– :: : infects a wild type (Su Kan s ) E. coli host, the phage DNA can not replicate ( PPPPP ) nor can it integrate ( i –– ) thus the only way for the E. coli to become Kan r is for TTTTT to transpose from the λ DNA to some location on the E. coli chromosome. This type of transposition is an inherently rare process and will occur in about one out of 10 5 phage-infected E. coli cells. This is how a transposon mutagenesis might be done: 1) Infect 2x10 9 wild-type E. coli cells with λ P – : so that each cell receives at least one phage chromosome. 2) Select for Kan r by plating on medium that contains kanamycin. There should be a total of about
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 11/05/2011 for the course BIOLOGY MCB2010 taught by Professor Jessicadigirolamo during the Fall '10 term at Broward College.

Ask a homework question - tutors are online