mmbio sg2

mmbio sg2 - Protein Structure 2.1 OVERVIEW Amino acids are...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Protein Structure 2.1 OVERVIEW Amino acids are the building blocks of proteins. Amino acids are linked by peptide bonds to form polypeptide chains. Protein folding is determined by weak non-covalent interactions among amino acid residues. Residues fold into higher order secondary structures, which then lead to the tertiary, three-dimensional fold of the protein. CONCEPT EMPHASIS 1. Amino acids. Amino acid side chains confer certain characteristic properties. 2. Peptide bonds. Peptide bonds link amino acids into polypeptide chains. 3. Weak non-covalent bonds. Non-covalent bonds stabilize secondary, tertiary, and quaternary protein structures. 4. Secondary structures. Protein secondary structures include "-helices, $-sheets, and loops and turns. 5. Tertiary structure. Tertiary structure is determined by x-ray crystallography or NMR. DETAILS 1. Amino acids have a cen 2 tral carbon atom attached to an amino (-NH ) group, a carboxyl (-COOH) group, a hydrogen atom, and a side chain (R). 2. Side chain R groups confer characteristic properties: - basic: Arg, Lys, His - acidic: Asp, Glu - polar, uncharged: Asn, Gln, Ser, Thr, Tyr, Cys - nonpolar: Gly, Ala, Ile, Leu, Val, Phe, Trp, Met, Pro 3. Peptide bonds are amide bonds between amino acids that link the carboxyl group of one amino acid with the amino group of the next amino acid. 4. Peptides are drawn with the amino (N) terminus to the left and the carboxyl (C) terminus to the right. 5. Column chromatography is a method to separate/fractionate proteins. 6. Ion exchange chromatography separates proteins by electrostatic interactions (charge) 7. Reverse phase chromatography separates proteins by hydrophobic interactions 8. Gel filtration (molecular exclusion) chromatography separates proteins by size 9. Affinity chromatography separates proteins by specific ligand binding properties
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 11/05/2011 for the course MMBIO 240 taught by Professor Bridgewater during the Fall '09 term at BYU.

Page1 / 3

mmbio sg2 - Protein Structure 2.1 OVERVIEW Amino acids are...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online