Ch04.1-26Sept

Ch04.1-26Sept - Chapter 4: Antigen Recognition by B-cell...

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Chapter 4: Antigen Recognition by B-cell and T-cell Receptors Monday 26 September 2011 This lecture was taken from: Chapter 4, pp. 127-153; Chapter 5, pp. 173-179; 186-190 Tutorial: Immunological methods (from the ImmunoBiology Appendix)
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Structure of a Typical Antibody Molecule Interaction of the Antibody Molecule with Specific Antigen Structural Variation in Immunoglobulin Constant Regions Evolution of the Adaptive Immune Response (sections 5-20 - 5-21) Monday 26 September This lecture was taken from: Chapter 4, pp. 127-140; Ch. 5, pp. 173-179; 186-190
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Figure 3-2
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Figure 3-1 part 3 of 3
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Figure 3-1 part 2 of 3 Immunoglobulin heavy and light chains form variable (V) and constant (C) domains N-termini H chain C-termini
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Figure 3-1 part 1 of 3
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Figure 3-1 part 2 of 3
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The Heavy Chain Constant Region Determines an Antibody’s Class/Isotype (and Subclass, if applicable). [Each constant region is separately encoded at the IgH locus.] Heavy chain constant region => Immunoglobulin (Ig) class/subclass μ = IgM; δ = IgD; γ 1,2a,2b,3 or γ 1-4 = m IgG 1,2a,2b,3 hu Ig 1-4 ; α = m IgA or hu IgA 1,2 ; ε = IgE Antibodies have either a hinge region + 3 C domains or no hinge region and 4 C domains.
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IgM + J chain is pentameric IgM - J chain is hexameric IgA + J chain is dimeric IgA - J chain is monomeric
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Figure 3-1 part 3 of 3
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Figure 3-3 Fab comprises the antigen-binding domain, and is used to determine “intrinsic” affinity of an antibody for an antigen (Kd). Fc comprises the “effector” domain of an antibody. It is bound by Fc receptors on cells, or soluble factors like C1q. F(ab’) 2 comprises bivalent Fab, and can be used to determine an antibody’s effect in the absence of its Fc-effector function. It also can be compared to Fab to gauge “apparent” affinity
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Figure 3-4 Different forms of immune complex are specified by: (i) the number of determinants on the antigen, (ii) the antibody isotype ( i.e., # of Fabs/Ig), and (iii) the ratio of antigen to antibody molecules (iv) the Kd of the antibody for the antigen, and their concentrations If antibody or antigen is in high molar excess, Ab-Ag complexes cannot form. “Multivalent” antigens, like a bacterial cell wall carbohydrate or DNA, have repeating
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Ch04.1-26Sept - Chapter 4: Antigen Recognition by B-cell...

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