430exam3_spring_2007 - Chem_430_Dr Hogan_s2007_Exam3 1...

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1 Print student name _______________________ Student PID________________________ Pledge_____________________________ Q1. When a mixture of glucose 6-phosphate and fructose 6-phosphate is incubated with the enzyme phosphoglucose isomerase, the final mixture contains twice as much glucose 6- phosphate as fructose 6-phosphate. Based on everything you know about metabolism and glycolysis in particular, which one of the following statements is most likely correct when applied to the reaction below ( R = 8.315 J/mol·K and T = 298 K)? Glucose 6-phosphate fructose 6-phosphate (1) ∆ G' ° is +1.7 kJ/mol. (2) G' ° is –1.7 kJ/mol. (3) G' ° is incalculably large and negative. (4) ∆ G' ° is incalculably large and positive. (5) G' ° is zero. Q2. The steps of glycolysis between glyceraldehyde 3-phosphate and 3-phosphoglycerate involve all of the following except: (1) ATP synthesis. (2)catalysis by phosphoglycerate kinase. (3)oxidation of NADH to NAD + . (4) the formation of 1,3-bisphosphoglycerate. (5) utilization of P i . Q3. The first reaction in glycolysis that results in the formation of an energy-rich compound (i.e., a compound whose hydrolysis has a highly negative G' °) is catalyzed by: (1)glyceraldehyde 3-phosphate dehydrogenase. (2)hexokinase. (3)phosphofructokinase. (4) phosphoglycerate kinase. (5) triose phosphate isomerase. Q4. Inorganic fluoride inhibits enolase. In an anaerobic system that is metabolizing glucose as a substrate, which of the following compounds would you expect to increase in concentration following the addition of fluoride? (1) 2-phosphoglycerate. (2)Glucose. (3)Acetyl CoA. (4)Phosphoenolpyruvate (5) Pyruvate Q5. Which of the following is not true of the reaction catalyzed by the pyruvate dehydrogenase complex? (1)Enzyme E1 catalyzes a reductive decarboxylation. (2)Both NAD + and a flavin nucleotide act as electron carriers. (3)The reaction occurs in the mitochondrial matrix. (4)Substrates are shuttled between enzymes E1 and E3 by the lipoamide function covalently attached to a residue of enzyme E2. (5) Two different cofactors containing —SH groups participate. Q6. Conversion of 1 mol of acetyl-CoA to 2 mol of CO 2 and CoA via the citric acid cycle (i.e., one complete “turn” of the TCA cycle) results in the net production of: (1) 1 mol of citrate. (2) 1 mol of FADH 2 . (3) 1 mol of NADH. (4) 2 mol of oxaloacetate. (5) 7 mol of ATP.
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This note was uploaded on 11/04/2011 for the course BIOL 430 taught by Professor Lspremulli during the Fall '08 term at UNC.

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430exam3_spring_2007 - Chem_430_Dr Hogan_s2007_Exam3 1...

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