Chapter 5_1

Chapter 5_1 - After chyrmotrypsin: IV PAGRAAF STVY MAVKLIW...

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After chyrmotrypsin: IV PAGRAAF STVY MAVKLIW We line up all the broken pieces LIWSTVYPAGR MAVK AAFIV MAVKLIW PAGRAAF IV After you have all these pieces, you run them through a database. VI. What do we do with the primary sequence information: Proteins that perform the same function in different organisms will have related sequences. a. Sequence alignment i. Invariant Regions 1. Regions that are critical to structure and function because they are conserved throughout evolution ii. Conservative substitutions 1. Positions in the primary sequence where one amino acid is switched with an amino acid of a similar sequence 2. Places were during the course of evolutionary time, there were slight changes. They do not have a net deleterious effect on the protein iii. Variable or hypervariable regions 1. Regions were the two sequences are different 2. They are not critical to structure and function Chapter 4 (p 97-109) : Protein Function We will use the examples of myoglobin and hemoglobin to discuss how protein tertiary and quaternary structure influence function. a. Myoglobin (Mb): 1 st protein whose crystal structure was determined 1. Structure a. Tertiary globular structure with 8 alpha-helical segments 2. Function a. Store oxygen in muscle i. The problem with oxygen is at room temp it is a gas; however it is a sparingly soluble gas – therefore this is used to do the job 3. Structural components a. Contains a heme ion, Fe 2+ , which is embedded in the porphoryn ring b. Iron is capable of making 6 coordinate covalent bonds, and this is important for myoglobin to bind oxygen 4. Oxygen binding curve of myoglobin
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a. Diatomic oxygen has to bind to the 6 th open bond in iron at a specific angle of 120 degrees – this has to do with the amino acid resides b. Myoglobin will also bind CO – it binds more tightly than O 2 c. The x axis is concentration of the pressure of oxygen d. The y axis is the degree of saturation e. It has a hyperbolic shaped curve – you only need a little oxygen to get myoglobin
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This note was uploaded on 11/04/2011 for the course BIOL 430 taught by Professor Lspremulli during the Fall '08 term at UNC.

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Chapter 5_1 - After chyrmotrypsin: IV PAGRAAF STVY MAVKLIW...

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