Problem_set_1 - r selection in this cross b You select 100...

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Problem set #1 – For Recitation the week of Feb 25 th . From Griffiths et al textbook: Chapter 5 (pg 215): problems 1, 2, 4, 6, 7, 8, 13, 14, 17, 20, 22, 26 Additional Problem: You mate an Hfr strain that is Ap s (sensitive to the antibiotic ampicillin), Gal + , Arg + , Lac - to a F - strain that is Amp r Gal - Arg - Lac + . You know that Gal is the last gene to be transferred by this Hfr strain and that Amp s is not transferred at all. a) What media would you plate the mating mix on to select for Amp r , Gal + Arg + progeny? What is the point of the Amp
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Unformatted text preview: r selection in this cross? b) You select 100 Gal + Ap r progeny and screen for the Arg & Lac markers. The following numbers of each phenotype are obtained (all are Gal + Ap r ). Arg + Lac + 20 Arg + Lac-75 Arg-Lac + 25 Arg-Lac-Draw a genetic map indicating the order of the Gal, Arg and Lac markers, and the crossover events leading to each phenotype (indicate which DNA is the recipient chromosome and which is the Hfr donor). (Do not need to indicate actual distances, just the order)....
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This note was uploaded on 11/04/2011 for the course BIOL 202 taught by Professor Kieber-hogan during the Fall '08 term at UNC.

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