Cell Cycle

Cell Cycle - Cell Cycle and Cancer The Cell Cycle Growth...

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Cell Cycle and Cancer
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START OR Restriction Point The Cell Cycle Growth factors G0
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MPF activity and cyclin protein expression during cell cycle Done in clam oocytes, in which cyclin is 5% of the total protein MPF is equivilent to cdc2 kinase (a key cyclin-dependent kinase)
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Yeast mutations identified a group of protein kinases called cyclin-dependent kinases (CDKs), whose activity is dependent on their association with the cyclins. That is, when CDKs are associated with cyclins, they are active, when not associated they are inactive. Negative charge of phosphate alters conformation, and thus activity of protein Phosphorylation reversible by protein phosphatases
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Expression of different Cdks and cyclins during cell cycle
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Cell Cycle Checkpoints G1 Checkpoint: “Is my DNA damaged?” G2 Checkpoint: “Is my DNA replicated?”
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Mitotic Checkpoint: “Are my chromosomes properly attached to the spindle?” Anti-tubulin antibody (mitotic spindle) DAPI stain (DNA)
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Cell Cycle Checkpoints
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G1 phase S phase cdk Growth Signal cyclin/ DNA damage, other insults p53 p21 How do cell cycle checkpoints work?
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PO4 Promotes degradation (Ub ligase) and excludes from nucleus ATM; CHK2 p53 expression is stimulated by sunlight, it begins the chain of events leading to tanning. Phosphorylation: 1) increases
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Cell Cycle - Cell Cycle and Cancer The Cell Cycle Growth...

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