lecture - cha 5-student

lecture - cha 5-student - CHAPTER 5 The Genetics of...

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CHAPTER 5 The Genetics of Bacteria and Their Viruses Copyright 2008 © W H Freeman and Company
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1. By what process do bacteria exchange genes? 2. Can these exchange processes be used to map genes producing mutant phenotypes? 3. How do phage genomes interact with bacterial genomes? 4. How can phage genomes be mapped? Key Questions
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5.1 Working with Microorganisms Plating: Colony: 10 7 Cell clone: Prototrophic: Minimum medium: basic requirements Auxotrophic: Resistant mutants
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5.2 Bacterial Conjugation Discovery of conjugation <-no, had to be in contact with each other
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5.2 Bacterial Conjugation Discovery of the fertility factor (F) Bacteria conjugate by using pili In prokaryotes, one source (donor) F factor (donor ability) -- plasmid state --integrated state- transmitted late in conjugation
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5.2 Bacterial Conjugation Hfr strains: On crossing with F - strains, Hfr strain produced 1000 times [recombinants] as many as a normal F none of the F - parents were converted into F + or into Hfr. Linear transmission of the HFR genes from a fixed point: Hfr azi r ton r lac + gal + str s x F - azi s ton s lac - gal - str r Interrupted mating. Mix F+ and F-, take them out at different times to give them different times to mate Exconjugants. [bacterial cell received a segment of the Hfr genom Gain b+ a+ wild type alleles for F- to turn into F+ it would have to receive the entire F+ chromosome plasmid {takes double crossover to switch one allele}
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5.2 Bacterial Conjugation Hfr strains: On crossing with F - strains, Hfr strain produced 1000 times as many as a normal F
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This note was uploaded on 11/06/2011 for the course BIL 250 taught by Professor Wang during the Fall '08 term at University of Miami.

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lecture - cha 5-student - CHAPTER 5 The Genetics of...

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