lecture - cha 8-student

lecture - cha 8-student - CHAPTER 8 RNA: Transcription and...

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CHAPTER 8 RNA: Transcription and Processing Copyright 2008 © W H Freeman and Company
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1. How does the structure of RNA differ from that of DNA? 2. What are the different classes of RNA in a cell? 3. How is RNA polymerase correctly positioned to start transcription? Key Questions
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4 . How is eukaryotic RNA modified before leaving the nucleus? 5. Why is the discovery of self-splicing introns considered to be so important? Key Questions
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8.1 RNA Evidence of RNA intermediate
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8.1 RNA Properties of RNA Unlike DNA can catalyze biological reactions
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8.1 RNA Classes of RNA Messenger RNA Code proteins, pass information for synthesis Functional RNA Transfer RNA (tRNA) Transfer amino acids Ribosome RNA rRNA Protein synthesis Small nuclear RNAs (snRNAs) splicing MicroRNAs (miRNAs) Regulation of gene expression, can degrade, protect integrity [prevent spread of transposable elements] Small interfering RNAs (siRNAs) Piwi-interacting RNAs Non-protein-coding RNA (ncRNA) DNA - Only in the nucleus, no enzymatic/catalytic activity RNA- catalytic activity
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8.2 Transcription DNA as transcription template RNA growth is always in the 5’ –to- 3’ direction; the template DNA strand is always oriented from 3’ to 5’. Can only use one segment of a region
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8.2 Transcription Overview of transcription RNA polymerase attaches to DNA and moves along it linking the aligned ribonucleotides
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8.2 Transcription Overview of transcription Many RNAs can be simultaneously transcribed from a gene. RNA bases in transcript and template are complementary, and is the same as that in the
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lecture - cha 8-student - CHAPTER 8 RNA: Transcription and...

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