bcmb8190_a9_10

bcmb8190_a9_10 - You would use a T2 enhanced sequence. T2...

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BCMB/CHEM 8190 ANSWERS PROBLEM SET 9 1) Dissociation constant = 2X10^-4 X 5X10^-6 = 1X10^-9 2) The relative rate of build-up of NOEs will be 300,000/2000. A 20% contribution from the free NOE would occur with a free to bound ratio of X/(X-1) where X*2)/((1- X)*300) = 0.2. Assuming the ligand binding constant is relatively high, 20% would occur at a 30:1 ligand to protein ratio. 3) Iron oxide nano particles exert their greatest effect on T2 – making it shorter.
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Unformatted text preview: You would use a T2 enhanced sequence. T2 filters usually result in loss of signal from regions having the shortest T2 these regions would appear dark. 4) a) dV = 1/( T2*) + 1/( ) = 0.5 + 1/( ) = 1.0; = 0.64s b) dV = 1/( T2*) + ( /(8 ))(wa-wb)**2 = 0.5 + ( /(8 ))(2 *100)**2 = 5.0; = 2.9x10**(-4)s...
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