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bcmb8190_p8_10

bcmb8190_p8_10 - ATP ribose and the methyl resonance of the...

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BCMB/CHEM 8190 PROBLEM SET 8 1) Below is a 15 N- 1 H HSQC spectrum of an RNA duplex. How many Gs and how many Us are in the molecule? 2) Below is a 1 H- 1 H NOESY spectrum of a self-complementary DNA decamer (GCGAATTCGC). A starting point is given. See if you can continue the assignment on the spectrum below.
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3) We collect a set of 50 1D 1 H spectra signal averaging each for 10 minutes beginning at a point where we add sodium acetate and ATP to a solution containing the enzyme acetyl-CoA synthase. We order the spectra from time zero to time 50 min in a matrix and process it as a STOCSY spectrum. a) If the resonances coming from the ATP molecules are connected by positive cross- peaks, what do you expect for the sign of a cross peak connecting the H1 resonance of the
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Unformatted text preview: ATP ribose and the methyl resonance of the acetyl-CoA product? b) What is the sign of the cross-peak connecting the AMP H1 and AMP H2 proton resonances? c) Would you expect a cross-peak to connect the H1 AMP resonance and the H8 AMP resonance? Would you expect to see this in a normal TOCSY spectrum? 4) For a DNP enhanced 13 C spectrum polarized at 4K and taken at 25C, what is the maximum enhancement, compared to a non-enhanced 13 C spectrum at 25C, coming from the DNP effect? What is the maximum enhancement coming from the temperature effect? What is the combined enhancement?...
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