BCMB8190MT001013

BCMB8190MT001013 - 4 P roto n l in e w idth s for n...

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4) P r o t on line widths for non-exchanging amide protons in myoglobin are approximately 15 Hz. Hemoglobin is a tetramer of subunits that are approximately the same size as myoglobin. What would you expect for line widths of non-exchangeable amide protons in hemoglobin? 5) We achieve a signal to noise ratio for a peak of interest of 3:1 after collecting 4 scans. What do you expect for the signal to noise ratio after 64 scans? /z.: ) 6) The coupling constant between two olefinic 13 C carbons in propene is 50 Hz (CH3-CH=CH2). What would you expect for the coupling between directly bonded 13 C and '"N nuclei in methyl cyanide (CH 3 -ON) and why? X - * ~> ~ ' 3*3" ^ Z 2 -' in ) / 9 lib- 9 5 / M * 7 'y /CL ^ - / ^ ^ / A x , ^ > ^ > _ Z _ ^ sg ^ - _ 4 _ y if ( J*ls»o<.Y S - ?^//^' -?,
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BCMB8190MT001013 - 4 P roto n l in e w idth s for n...

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