COSY&TOCSY_10 - COSY and TOCSY BCMB/CHEM 8190 COSY...

Info iconThis preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon
COSY and TOCSY BCMB/CHEM 8190
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
COSY for an AX Spin System Hamiltonian: H = - γ B 0 (1- σ A ) I ZA - γ B 0 (1- σ X ) I ZX + 2 π J I ZA · I ZX chemical shift scalar coupling Basis set : ( αα , αβ , βα ββ ) Using product operator transformation tables, or Kanters’ POF procedures for MAPLE: step1:=spinsystem([A,X]); step2:=xpulse(step1,{A,X},Pi/2); d1 (recover) t1 (evolve) t2 (observe) 90x 90x (mix)
Background image of page 2
> step3:=evolve(step2,{A,X}, t1); Evolution Step Combines All parts of Hamiltonian in Kanters’ POF Approach
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Now the effect of another X pulse: I XA + I XX > step4:= xpulse(step3, {A,X}, Pi/2); some of the above are not observable (MQ,Z) – only retain observables some X observables were modulated by properties of A in t1 – crosspeaks some X observables were modulated by properties of X in t1 - autopeaks
Background image of page 4
Observe: take the trace of result with Mx and My operators ( I XA + I XX + I YA + I YX ). I is Y component > step5:=observe(step4, {A,X}, t2,0); Note: t1 must reach ~1/(2J) or sin terms make crosspeaks small
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Useful Trigonometric Identities: • sin(A)cos(B) = ½ [sin(A+B) + sin(A-B)] • cos(A)sin(B) =
Background image of page 6
Image of page 7
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 11/07/2011 for the course CHEM 8853R taught by Professor Gelbaum during the Fall '11 term at Georgia Institute of Technology.

Page1 / 17

COSY&TOCSY_10 - COSY and TOCSY BCMB/CHEM 8190 COSY...

This preview shows document pages 1 - 7. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online