Metabonomics_10

Metabonomics_10 - Metabonomics and MRS BCMB/CHEM 8190...

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Unformatted text preview: Metabonomics and MRS BCMB/CHEM 8190 Definition: The quantitative measurement of the dynamic multi parametric metabolic response of living systems to physiological stimuli or genetic modification. ( J.K. Nicholson, J.C. Lindon, E. Holmes, Xenobiotica 29, 1181-1189, 1999) What do you observe? Metabolites the small molecule (< 1500 Da) substrates and products of the complex enzyme networks that support life Why study metabolomics? Metabolite variations are the end product of the action of proteins, which are the end product of gene expression, they correlate more directly with disease, toxicological effects, and environmental variations. References: Wishart, D. S. (2008). Applications of metabolomics in drug discovery and development. Drugs in R&D 9 , 307-322. Lindon, J. C., Holmes, E. & Nicholson, J. K. (2007). Metabonomics in pharmaceutical R & D. Febs Journal 274 , 1140-1151 Metabolomics, Metabonomics, Metabolic Profiling Advantages High Sensitivity (pico grams) Observe a diverse number of molecular species Simple relationship between observable and molecule: a direct measure of molecular weight Limitations Limited molecular structure detail Requires pre-analysis separation Quantitation is relative and usually requires isotopic labeling Some metabolites are difficult to detect because of ionization difficulties Observation of Metabolites Mass Spectrometry (MS) Nuclear Magnetic Resonance (NMR) Advantages Real-time application to many systems without pre-treatment Quantitative response to concentrations of metabolites...
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Metabonomics_10 - Metabonomics and MRS BCMB/CHEM 8190...

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