Product_Operator_Applications_10

Product_Operator_Applications_10 - Product Operator...

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Product Operator Applications: 2D Correlation Experiments: HSQC, HMQC, HMBC, BCMB/CHEM 8190
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Two Dimensional NMR Spectra A General Scheme: other mixing and evolution periods can be added to increase dimensions Preparation Evolution 1 (Increment t1) Mixing Evolution 2 (observe t2) time Example: COSY – mixing is scalar coupling d1 (recover) t1 (evolve) t2 (observe) 90x 90x (mix)
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2D –NMR - FIDs are Transformed in t2, then in t1 ν 2 t1 t1 ν 2 ν 1 FT
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Heteronuclear Single Quantum Coherence HSQC 1 H- 15 N Pairs in Amide Bonds of Proteins Provide Convenient Reporters at Each Amino Acid Along the Backbone Just Requires 15 N Labeling
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HSQC Spectrum of H-N Amides in a Protein Phosphoinositide interactions with PH domain of FAPP1 at a bicelle surface Red – without PI(4)P Blue – with PI(4)P
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15 N 1 H Heteronuclear Magnetization Transfer as Used in the INEPT and HSQC Experiments 1 H(I) 15 N(S) Iz -Iy -2IxSz -2IzSy 90x 180y 90y 90x τ τ τ = 1/4J
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Evolution and Detection in HSQC t1/2
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Product_Operator_Applications_10 - Product Operator...

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