Cell And Tissue Culture

Cell And Tissue Culture - Cell And Tissue Culture Mammalian...

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Cell And Tissue Culture Mammalian Cells Advanced Higher Biology
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Why is it useful? Gene manipulation Culturing mammalian cells for cancer studies Producing new plants through tissue culture
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What do you need to do it? Source of cell material -freshly prepared -stock of cell line -bacterial culture
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Suitable container Simple flask Sophisticated fermenter with computer- controlled monitoring
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Growth medium Glucose Water Amino acids Salts
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Opportunity for Gas Exchange Oxygen Carbon dioxide
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Animal serum Foetal Bovine Serum Essential for animal cell proliferation 5% - 10% of growth media
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Indicator Waste products causes change in pH Use indicator like phenol red Changes from red to yellow
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Control of Temperature and pH 37.5 O C pH 7.5
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Method for Measuring Cell Growth Counting cell numbers in culture (haemocytometer) Measure optical density in spectrophotometer
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Sterilisation Antibiotics Sterilisation
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Anchorage – dependant
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This note was uploaded on 11/07/2011 for the course BIOLOGY 100 taught by Professor Staff during the Winter '08 term at BYU.

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Cell And Tissue Culture - Cell And Tissue Culture Mammalian...

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