labrespiration1

labrespiration1 - CELL RESPIRATION Introduction to cell...

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Unformatted text preview: CELL RESPIRATION Introduction to cell respiration laboratory This laboratory consists of three sets of experiments that illustrate the oxidation of glucose for the production of ATP. 1.) The photosynthesis vs. respiration experiment illustrates the importance of respiration to plants. 2.) Fermentation products will be tested for to demonstrate the importance of yeast in the fermentation process. 3.) An enzymatic reaction of the Krebs cycle will be studied to show how enzyme activity may be manipulated by changing substrates. CELL RESPIRATION Introduction to comparison of photosynthesis and respiration It is well known that in the presence of light plants perform photosynthesis. It is less well understood that at the same time plants are also performing cell respiration. To demonstrate this we will determine whether CO 2 is consumed or produced as Elodea is placed in either a light or dark environment. The change in CO 2 will be detected by the pH indicator phenol red. Think about the chemical equations for respiration and photosynthesis. Which one releases CO 2 causing an increase and which one uses up CO 2 which will cause a decrease? CELL RESPIRATION Introduction to comparison of photosynthesis and respiration (cont.) Phenol red is yellow under acidic conditions ( high H ion concentration ), pink under basic or alkaline conditions ( low H ion concentration ) and orange under neutral conditions. A change in CO 2 will cause a directly proportional change in H ion. If the CO 2 concentration decreases, the H ion concentration will also decrease and the solution will change to pink . If the CO 2 concentration increases, the H ion concentration will also increase and the solution will change to yellow . CELL RESPIRATION Experimental procedure for comparison of photosynthesis and respiration Step 1: Fill four test tubes approximately 2/3 full with tap water and add 15 drops of phenol red. What tap water should turn pink. Is Glendora tap water acidic or basic?...
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labrespiration1 - CELL RESPIRATION Introduction to cell...

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