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Unformatted text preview: BIO 325 Genetics Homework 2 This homework 2 is due Thursday, Jun. 16 Please turn in your homework to your TA (NMS 3.276) or bring it to the class Please don't try to write on this sheet! Put your answers on another sheet of paper and turn that in. (Q 1-2) You are studying a mutation in the yeast Saccharomyces cerevisiae that is temperature sensitive (no growth above 32 degrees). This mutation (which you name mft2 for my favorite temperature sensitive 2) may be a new gene and you want to investigate. First, you cross your haploid mft2 strain to a haploid strain of the opposite mating type carrying trp1 and yfg4, both recessive markers on chromosome IV. The phenotype of trp1 is a requirement for tryptophan for growth and this gene is very tightly linked to the centromere. The phenotype of yfg4 is temperature-sensitivity. However, in this case, there is no growth above 28 degrees. At 30 degrees, yfg4 cells will not grow but mft2 cells grow normally....
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This note was uploaded on 11/07/2011 for the course BIO 325 taught by Professor Saxena during the Summer '08 term at University of Texas.
- Summer '08