Bio for non majors 14

Bio for non majors 14 - Automated DNA sequencing Contains...

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Forensic Biotechnology Review Short Tandem Repeat (STR) analysis Y chromosome analysis Mitochondrial DNA analysis PCR amplify each hyper-variable region Perform sequence analysis Sanger sequencing Automated sequencing Sanger Sequencing Uses dideoxynucleotides to terminate newly synthesized DNA fragments at specific bases 4 separate reactions each with: Labeled primer DNA polymerase 4 bases 1 dideoxy base Produces a collection of DNA strands of different lengths Run each reaction separately on a gel
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Unformatted text preview: Automated DNA sequencing Contains all 4 bases and all 4 dideoxy bases, each with a different in fluorescent label one reaction Run the reaction on a gel Electronic DNA sequence trace Key Points How are the following forensic analyses performed and what are the advantages of each? Short Tandem Repeat (STR) analysis Y chromosome analysis Mitochondrial DNA analysis Sanger DNA sequencing vs. automated sequencing...
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This note was uploaded on 11/08/2011 for the course BIO 0815 taught by Professor Crucielli during the Spring '11 term at Pittsburgh.

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