BIO 3465 - Lab 2 Structure of Plant Cells _ Tissues

BIO 3465 - Lab 2 Structure of Plant Cells _ Tissues - BIO...

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BIO 3465 Plant Physiology Laboratory 2 STRUCTURE OF PLANT CELLS AND TISSUES Goal: The cell is the basic functional unit of any living organism. Cell structure is closely related with its function. In this laboratory exercise you will learn the basic structure of a plant cell, structural differences among different cell and tissue types and how these structural differences are related their functions. Mostly, you will be examining prepared slides using compound microscopes. Additionally, you will also prepare your own specimens to get hand on experience. INTRODUCTION: The cell is the fundamental unit of structure and function in living organisms. Thus, all living organisms composed of cells. Certain organisms such as bacteria, yeast, diatoms, and dinoflagellates are made of single cells ( unicellular organisms ), other organisms consist of many cells ( multicellular organisms ). While unicellular organisms are capable of performing all the living functions in a single cell, cells of most multicellular organisms are specialized for different functions. The structure of a cell is intimately associated with its function. Thus, multicellular organisms are made up many cell types. Each cell type performs a different function. Their functions may be either physiological (such as transport of water, gas exchange) or biochemical (such as photosynthesis, respiration) or both physiological and biochemical functions. Most of the plants you study in this course such as ferns, moss, and flowering plants are multicellular organisms. Almost all multicellular plants (except the plants that are propagated exclusively by vegetative means) begin their life cycle as a single diploid cell called ‘zygote’. This single cell divides mitotically to produce genetically identical, but either similar or dissimilar cells. Cells that are different from each other produce different tissues. Similar cells group together to produce a particular tissue with a specific function. In this way different cells produce different tissues with specific functions. As function of a tissue is closely related to it structure, in a multicelluar organism many different types of tissues can be identified. All these tissues originate from groups of non- specialized cells called meristems . Multicellular plants are made up of many different types of cells and tissues. These different cells compose of different structures that enable them to carry out specific functions. Despite the differences among cell types, all of these cells possess common features such as the presence of cell wall, vacuole, nucleus, ribosomes, endoplasmic reticulum and other membrane bound organelles etc.
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PART I. Cell Ultrastructure A. Observing Living Cells with Light Microscopy Procedure: 1. Aquatic Leaf ( Hydrilla or some other aquatic plant may be used) You are supplied with freshly collected aquatic plant. Remove a relatively younger leaf from the stem. Prepare a wet mount of the entire leaf. Examine cells of the leaf using the compound microscope. Look at the shape of individual cell. What are the organelles you can recognize
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BIO 3465 - Lab 2 Structure of Plant Cells _ Tissues - BIO...

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