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Lecture 11 - 11 Information Transfer IV DNA Replication...

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11. Information Transfer IV DNA Replication. Iain McKinnell Dept Biology
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Information Transfer Why do cells divide? Reproduce the organism (prokaryotes). If the organism is eukaryote, cells divide to reproduce organism (unicellular yeasts), replace dead cells or to allow the organism to grow. http://www.brown.edu/Course s/BI0032/repro/malecon.htm http://www.flickr.com/photos/pinksto ckphotos/5264093772/in/photostream/ Cell growth & division: Total DNA Replication
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Cell division in either prokaryotes or eukaryotes must be accompanied by replication of DNA
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How does DNA replicate? DNA = genetic material = hereditary molecule. Complimentary base pairing is key explains how DNA replicates.
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DNA Replication Watson & Crick imagined a multi-step process: Hydrogen bonds break Strands unwind & separate Each strand acts as a template
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Mode of Replication
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Meselson-Stahl Experiment http://www.nature.com/nature/journal/v417/n688 5/full/417121a.html
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Meselson-Stahl Experiment
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Mode of Replication
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Mode of Replication: Meselson- Stahl experiment.
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DNA Replication
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Assembling a Complementary Chain Polymers of nucleotides. Three parts (covalent bonds) Base (carbon & nitrogen atoms) Pentose (five carbon) sugar Phosphate group/s
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Assembling a Complementary Chain Rules 1)DNA is produced from DNA by DNA Polymerase DNA is the template; complementary DNA is the product 2)DNA chain growth is 5’ to 3’ - must add to preexisting polynucleotide strand Mediated by base pairing
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DNA Replication
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