Lecture 13 - 13. Genetics I Recombination Iain McKinnell...

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13. Genetics I Recombination Iain McKinnell Dept Biology
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Midterm Wednesday 9 th November Written during usual class period (11:35-12:55) If your surname starts with A- C you need to go to Tory 110 (your lab room) for your exam. Everyone else (Surnames from D Z), just come to AT301 as usual.
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Midterm 40 multiple choice questions. Just the material covered in the time between Midterm I and today - Chapters 9, 13-15. Bring a pen & pencil(s) scantron sheets + question booklet (write name, student number, exam version on both both must be handed in). No electronics (phones, Ipods etc) or notes. If missed have to provide medical documentation.
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Result of binary fission - prokayotes Cell division yields progeny that are identical to parental cell except for mutations that occur during cellular reproduction
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Result of mitotic cell division - eukaryotes Cell division yields progeny that are identical to parental cell except for mutations that occur during cellular reproduction Example: Somatic cells in humans Genetic information in an embryo is conferred to trillions of cells that comprise the body The genetic information is the same in each cell , but different cells express the information differently
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Genetic diversity 1) Two parents give rise to unique combinations of genetic material during fertilization. 2) Sexual reproduction gives rise to greater variation than simple cell division Parents are different from offspring Basis of variation lies in RECOMBINATION of genetic material Eukaryotes - a process called meiosis Prokaryotes….
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Mechanism of Genetic Recombination Requires 2 DNA molecules that differ from one another in at least 2 places Homology allows DNA on different molecules to line up and recombine precisely Enzymatic cutting and pasting of both DNA backbones from each of 2 DNA molecules required for recombination 2 circular molecules “fuse” together
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Genetic Recombination in E. coli E. coli with mutagens no longer grow on minimal medium ( auxotroph ) Vitamin biotin and amino acid methionine Amino acids leucine and threonine, vitamin thiamine Three letter gene name, + normal, mutated allele If combined strains grew on minimal medium, then recombination occurred Several hundred out of 100 million grew
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Replica Plating Replica plating identifies and counts genetic recombinations in bacterial colonies Master plate pressed onto sterile velveteen Velveteen pressed onto replica plates with different
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This note was uploaded on 11/08/2011 for the course BIOL 1103 taught by Professor Mckinnel during the Fall '11 term at Carleton CA.

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Lecture 13 - 13. Genetics I Recombination Iain McKinnell...

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