BE 167L Lecture 2 General (ppt)

BE 167L Lecture 2 General (ppt) - Lecture 2 Brief...

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Lecture 2
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Brief discussion of optics and resolution Optical, fluorescent, and confocal microscopy Further Reading: http://www.microscopyu.com/articles/optics/components.html Next week! Fluorophores for fluorescent microscopy Bioconjugation New techniques
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Key Components: • Illumination source • Light conditioner • Condenser • Objective • Sample • Eyepiece • Camera
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Table 1 δ = f sin( α) Snell’s Law of Refraction
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d f M h h T = = ) 1 ( / ) 2 ( magnification h(1) h(2) d
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Most common – Köhler Illumination Uniform collimated illumination – light is focused on focal point of condenser
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Why can’t magnify something to infinity? Quick answer: diffraction Abbe’s Theory of Image Formation Object acts as a transmission grating – the more “orders” captured the better the image
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Resolvable distance between two point sources: eff NA q 2 22 . 1 1 λ = θ sin , 2 n NA NA NA NA o c eff = + = n = index of refraction of medium (e.g. air, oil, water) λ = wavelength of light Condenser, NAc Objective, NAo
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BE 167L Lecture 2 General (ppt) - Lecture 2 Brief...

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