BE 167L Lecture 3 General (ppt)

BE 167L Lecture 3 General (ppt) - Lecture 3 Fluorescence...

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Unformatted text preview: Lecture 3 Fluorescence and fluorophores Reactivity of Amino Acids Standard Bionconjugation Chemistries Example: Attaching Fluorophores to Proteins Next Lecture Polymerization Soft lithography Micro-contact Printing Fluorescein DAPI - 4',6-diamidino-2-phenylindole Rhodamine Key point: fluorophores in visible wavelengths are highly conjugated λ / hc hv E = = Photons are absorbed Electrons are vibrationally excited They relax to lowest excited state They emit a photon of lower energy Franck-Condon Principle – No molecular movement during photon absorption Relaxation is faster than emission, leading to the Stokes Shift nr k Q + Γ Γ = Quantum yield, ratio of number of photons emitted to absorbed, Rate of radiative decay, Γ Non-radiative decay, k nr Solvent dielectric properties Temperature Nearby molecules affecting energy of excited or ground state Neighboring absorbing molecules (Resonance Energy Transfer – RET or FRET) Quenching – Oxygen and other...
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This note was uploaded on 11/10/2011 for the course BE 167 taught by Professor Staff during the Spring '10 term at UCLA.

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BE 167L Lecture 3 General (ppt) - Lecture 3 Fluorescence...

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