BE 167L Lecture 3 General (ppt)

BE 167L Lecture 3 General (ppt) - Lecture 3 Fluorescence...

Info icon This preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 3
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Fluorescence and fluorophores Reactivity of Amino Acids Standard Bionconjugation Chemistries Example: Attaching Fluorophores to Proteins Next Lecture Polymerization Soft lithography Micro-contact Printing
Image of page 2
Fluorescein DAPI - 4',6-diamidino-2-phenylindole Rhodamine Key point: fluorophores in visible wavelengths are highly conjugated
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
λ / hc hv E = =
Image of page 4
Photons are absorbed Electrons are vibrationally excited They relax to lowest excited state They emit a photon of lower energy Franck-Condon Principle – No molecular movement during photon absorption Relaxation is faster than emission, leading to the Stokes Shift nr k Q + Γ Γ = Quantum yield, ratio of number of photons emitted to absorbed, Rate of radiative decay, Γ Non-radiative decay, k nr
Image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Solvent dielectric properties Temperature Nearby molecules affecting energy of excited or ground state Neighboring absorbing molecules (Resonance Energy Transfer – RET or FRET) Quenching – Oxygen and other
Image of page 6
Image of page 7
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern