BE 167L Lecture 6 General

BE 167L Lecture 6 General - BE 167L – Bioengineering Lab...

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Unformatted text preview: BE 167L – Bioengineering Lab Lecture 6 Today • 2D vs. 3D Cell Culture and the cell microenvironment – 2D Culture – 3D Culture – Literature examples • 3D encapsulation for lab next week Tuesday Mammary Branching Morphogenesis Case Study Why 2D / 3D Cultures? 2D Cultures • Simple to grow and feed cells • Easy to image cells with microscopy 3D Cultures • Understanding cell behavior with in vivo relevance • Develop scaffolds for tissue engineering • Create environments to differentiate stem cells to desired lineages Lippincott-Schwartz Lab Input-Output Response of a Cell Yarmush & King. Annual Review Biomed Engineering 2009 3D 3D 2D The Cell Microenvironment (2D) Soluble Factors • Cytokines • Growth Factors • Hormones • Nutrients Interleukin-8 (IL-8) Epidermal Growth Factor (EGF) Epinephrine Cell-ECM Contact ECM – Extracellular Matrix Protein Components: Laminin, Collagen, Elastin, Fibronectin Proteoglycan Components: Heparan Sulfate, Chondroitin Sulfate Sugar Components: Hyaluronic Acid Cell-Cell Communication...
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This note was uploaded on 11/10/2011 for the course BE 167 taught by Professor Staff during the Spring '10 term at UCLA.

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BE 167L Lecture 6 General - BE 167L – Bioengineering Lab...

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