BE 167L Lecture 8 General

BE 167L Lecture 8 General - Types of co-culture Bhatia et...

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BE 167L – Bioengineering Lab Lecture 8
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For Lab Developing your own lab protocol for patterned cell culture!
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Today Co-culture: cell cultures with multiple cell types Building tissues and understanding signaling Isolating soluble vs. contact factors Selective cell adhesion: patterning multiple cell types Patterning DNA and proteins - Microarrays Next Tuesday Case Study 6: ECM microarrays
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Why Co-culture? Building tissues Uncover intercellular signaling mechanisms www.drexel.edu engineered
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Soluble Factors Conditioned Media Contains autocrine/paracrine factors at high levels Contains waste Will not allow investigation of factors that change dynamically with time Only one-way signaling Transwell Plates Transfer of autocrine/paracrine factors – transport should be controlled Dynamics can be investigated with care Two-way signaling can be investigated * Note: no cell-cell contact signaling with these methods
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Soluble factors only (Transwell) Cell-cell contact Random Micropatterned
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Unformatted text preview: Types of co-culture Bhatia et al. FASEB J 1999 Random mixed cultures Important Interactions Cell-cell interface-Homotypic vs. Heterotypic Interactions Cell ratio # / # * Note: Cannot be independently controlled OR Patterned mixed cultures Bhatia et al. FASEB J 1999 Controlled cell ratio, different heterotypic / homotypic ratios Differences in Albumin Expression Monoculture Co-culture Day 1 Day 6 Differential adhesion to ECM for co-culture Fukuda et al. Biomaterials 2006 mESC + hepatocyte co-culture Lee et al. Integrative Biology 2009 Made with a DNA microarraying robot Co-culture differentiation FITC - collagen Fibronectin Alkaline Phosphatase Embryonic stem cell spreading ES cells showed hepatocyte like transcription profiles Questions raised by these experiments? DNA Microarrays Affymetrix photolithographic DNA Microarrays Polymerase Chain Reaction Primers Taq Polymerase Nucleotides (dATP, dTTP, dCTP, dGTP) Temperature...
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This note was uploaded on 11/10/2011 for the course BE 167 taught by Professor Staff during the Spring '10 term at UCLA.

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BE 167L Lecture 8 General - Types of co-culture Bhatia et...

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