BE 167L Lecture 9 General

BE 167L Lecture 9 General - Primers Taq Polymerase...

Info iconThis preview shows pages 1–12. Sign up to view the full content.

View Full Document Right Arrow Icon
BE 167L – Bioengineering Lab Lecture 9
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Today Patterning of pluripotent stem cells affects differentiation trajectories Patterning DNA and proteins – Microarrays Cell – Extracellular matrix (ECM) interactions LAST Case Study next Tuesday
Background image of page 2
Controlling stem cells Discher, Mooney, Zandstra Science 2009
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Liu Bowens, Zandstra et al. Stem Cells 2008 Patterning stem cell Ebs Micropatterning creates uniform Ebs uniform sizes promote uniform behavior
Background image of page 4
Oct4 (red) – transcription factor representative of pluripotency Gata6 (green) – endoderm marker Ratios change with EB size. EB size affects fate
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
DNA Microarrays Affymetrix – photolithographic DNA Microarrays
Background image of page 6
Polymerase Chain Reaction
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 8
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 10
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 12
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Primers Taq Polymerase Nucleotides (dATP, dTTP, dCTP, dGTP) Temperature Reverse transcription is generally done before PCR Cell ECM Interactions Focal Adhesions Focal Complexes Fibrillar Adhesions Podosomes Geiger et al. Nat Rev Cell Biol . 2001 GTPases Precursors to focal adhesions Anchor cyoskeleton to substrate through large (1-2 um) protein complexes Cylindrical structures contain focal proteins macrophages + osteoclasts Integrins Alpha beta Heterodimers Alpha 5, beta 1 Interacts with Fibronectin Alpha V, beta 3 Vitronectin Layilin Hyaluronan Binding protien FAs reinforced with applied force...
View Full Document

This note was uploaded on 11/10/2011 for the course BE 167 taught by Professor Staff during the Spring '10 term at UCLA.

Page1 / 12

BE 167L Lecture 9 General - Primers Taq Polymerase...

This preview shows document pages 1 - 12. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online