BE 167L Lecture 9 General

BE 167L Lecture 9 General - Primers Taq Polymerase...

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BE 167L – Bioengineering Lab Lecture 9
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Today Patterning of pluripotent stem cells affects differentiation trajectories Patterning DNA and proteins – Microarrays Cell – Extracellular matrix (ECM) interactions LAST Case Study next Tuesday
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Controlling stem cells Discher, Mooney, Zandstra Science 2009
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Liu Bowens, Zandstra et al. Stem Cells 2008 Patterning stem cell Ebs Micropatterning creates uniform Ebs uniform sizes promote uniform behavior
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Oct4 (red) – transcription factor representative of pluripotency Gata6 (green) – endoderm marker Ratios change with EB size. EB size affects fate
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DNA Microarrays Affymetrix – photolithographic DNA Microarrays
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Polymerase Chain Reaction
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Unformatted text preview: Primers Taq Polymerase Nucleotides (dATP, dTTP, dCTP, dGTP) Temperature Reverse transcription is generally done before PCR Cell ECM Interactions Focal Adhesions Focal Complexes Fibrillar Adhesions Podosomes Geiger et al. Nat Rev Cell Biol . 2001 GTPases Precursors to focal adhesions Anchor cyoskeleton to substrate through large (1-2 um) protein complexes Cylindrical structures contain focal proteins macrophages + osteoclasts Integrins Alpha beta Heterodimers Alpha 5, beta 1 Interacts with Fibronectin Alpha V, beta 3 Vitronectin Layilin Hyaluronan Binding protien FAs reinforced with applied force...
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