{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Lab 2 Protocol 167L

Lab 2 Protocol 167L - Bioengineering 167L Lab 2 Process NHS...

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Bioengineering 167L Lab 2 Process: NHS conjugation and fluorescence microscopy Required Equipment: Stir plate, fluorescence microscope Chemicals: Chicken serum albumin (~45kDa) NHS-fluorescein Bead suspension Supplies: glass vials, slide glass, cover slip PURPOSE: To conjugate fluorescein, a fluorescent molecule, onto chicken serum albumin. Later in this course you will be carrying out tasks where fluorescence microscopy is required. Here you will learn the fundamentals of this technique and the software available. A guide to the Nikon Eclipse TS100 and its accompanying software is available in the lab and on courseweb (Teaching Lab Equipment Guides: Nikon Eclipse TS100 (Fluorescence Microscopy). For building familiarity with the equipment and the software you will take several images of green fluorescent microparticles of unknown sizes and measure their sizes. For a more thorough introduction to fluorescence microscopy please visit http://www.microscopyu.com/articles/fluorescence/fluorescenceintro.html. PROCEDURE: NHS conjugation 1. Prepare 15mL of a 5mg/mL albumin solution in phosphate buffered saline (PBS) in a glass vial. 2. Calculate amount of NHS-fluorescein reagent to add to the solution.
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern