Lab 2 Protocol 167L

Lab 2 Protocol 167L - Bioengineering 167L Lab 2 Process:...

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Bioengineering 167L Lab 2 Process: NHS conjugation and fluorescence microscopy Required Equipment: Stir plate, fluorescence microscope Chemicals: Chicken serum albumin (~45kDa) NHS-fluorescein Bead suspension Supplies: glass vials, slide glass, cover slip PURPOSE: To conjugate fluorescein, a fluorescent molecule, onto chicken serum albumin. Later in this course you will be carrying out tasks where fluorescence microscopy is required. Here you will learn the fundamentals of this technique and the software available. A guide to the Nikon Eclipse TS100 and its accompanying software is available in the lab and on courseweb (Teaching Lab Equipment Guides: Nikon Eclipse TS100 (Fluorescence Microscopy). For building familiarity with the equipment and the software you will take several images of green fluorescent microparticles of unknown sizes and measure their sizes. For a more thorough introduction to fluorescence microscopy please visit http://www.microscopyu.com/articles/fluorescence/fluorescenceintro.html. PROCEDURE: NHS conjugation 1. Prepare 15mL of a 5mg/mL albumin solution in phosphate buffered saline (PBS) in a glass vial. 2.
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This note was uploaded on 11/10/2011 for the course BE 167 taught by Professor Staff during the Spring '10 term at UCLA.

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Lab 2 Protocol 167L - Bioengineering 167L Lab 2 Process:...

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