practice review for quiz 1 sample answers

practice review for quiz 1 sample answers - Provided below...

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Provided below are some possible answers to the practice quiz. Many times there are multiple ways to answer a question. As long as you justify your reasoning and back it up correctly, you can receive full credit for many different answers. You are studying human dermal fibroblasts under conditions which should cause them to proliferate and express many extracellular matrix (ECM) proteins. They are being grown on porous polymer sheet scaffolds so that they make relatively thin (0.2mm) sheets which are 1cmx1cm square. You want to be able to measure cell proliferation by DNA assay and production of some ECM proteins by antibody labeling over the course of several weeks. 1. how do you create your samples so that each one is an INDEPENDENT sample? Make each film independently that the cells are cultured on, or repeat the experiment on different days with fresh batches of medium and newly thawed cells. 2. How many samples should you make in order to test for proliferation, collagen type I production, and elastin production, why? At least 3 for each test, which makes 9. A reliable student t-test requires at least 3 independent samples for each condition. More realistically you should make more than 3 in case something happens to a few of them, so maybe 15 total is also a fine answer. 3. How many time points should you plan for and what would it be based on? I would have to look up other experiments on this cell type to see how long it usually takes them to produce measurable changes in the ECM proteins I’m measuring and their proliferation rate. I would take at least 3 different time points, a 0 time point, a point in the middle, and a point a little
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practice review for quiz 1 sample answers - Provided below...

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