Exp_0_KEY_S11

Exp_0_KEY_S11 - KEY EXP 0 CALCULATIONS AND POINTS FOR...

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KEY EXP 0: CALCULATIONS AND POINTS FOR DISCUSSIONS ( entered values are samples ) 1. Report the best results from Table 0-1 in the table below: Values such as 0.225 or 0.236 are usually a result of the cuvettes being placed into the spec in the wrong direction. Values consistently above 0.55 (such as 0.59 or 0.62) are most likely due to pushing the plunger on the pipette too far before drawing up the BPB 2. Attach the printed spectrum of the bromphenol blue solution. (i) Was 591 nm the best wavelength choice to measure dye absorbance in the exercise? Yes, the peak maximum for BPB was 591 nm + 1 nm (ii) What other wavelength(s) could be used? Theoretically, any wavelength under the peak can be use, but best to limit range between ~450 nm to ~615 nm where there is sufficient absorbance to measure, and more importantly, to measures changes in absorbance as the concentration of BPB changes. (iii) Is 595 nm a good wavelength for measuring bromphenol blue?
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This note was uploaded on 11/10/2011 for the course MCB 120L taught by Professor Fairclough during the Spring '08 term at UC Davis.

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Exp_0_KEY_S11 - KEY EXP 0 CALCULATIONS AND POINTS FOR...

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