Answers Quiz two

Answers Quiz two - 1 Answers are in red under each question 1(14 points A patch of membrane containing-adrenergic receptors and other relevant

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1 Answers are in red under each question. 1. (14 points) A patch of membrane containing β -adrenergic receptors and other relevant membrane proteins was pulled from a cell using a glass pipette, similar to the process used in a patch clamping technique. As shown in the diagram below, the external surface of the membrane is in contact with the solution in the pipette and the cytoplasmic surface of the membrane is immersed in a small well. The well contains buffer that is equivalent to the salt composition (Mg ++ , Ca ++ , etc.) of the cytosol and contains ATP, but does not contain other small molecules such as other nucleotides. intact cell pipette - - well outer solution inner solution pipette - Various substances were added to either the solution in the bore of the pipette (outer solution) or to the solution in the buffered well (inner solution). The solution in the well (inner solution) was then assayed for cyclic AMP. This system was used to conduct several experiments numbered in the Table below. The outcome of experiments
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This note was uploaded on 11/10/2011 for the course BIS 104 taught by Professor Scholey during the Spring '08 term at UC Davis.

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Answers Quiz two - 1 Answers are in red under each question 1(14 points A patch of membrane containing-adrenergic receptors and other relevant

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