Certain restriction enzymes leave dangling ends of DNA molecules at the point where the DNA is opene

Certain restriction enzymes leave dangling ends of DNA molecules at the point where the DNA is opene

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Certain restriction enzymes leave dangling ends of DNA molecules at the point where  the DNA is opened. Foreign DNA can therefore be combined with the carrier DNA at this  point. An enzyme called DNA ligase forges a permanent link between the dangling ends  of the DNA molecules at the point of union. Recombinant DNA technology is sophisticated and expensive. Genes must be isolated,  vectors must be identified, and gene control must be maintained. Stability of the vector  within a host cell is important, and the scientist must be certain that nonpathogenic  bacteria are used. Cells from mammals can be used to synthesize proteins, but 
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Unformatted text preview: cultivating these cells is difficult. In addition, the proper gene signals must be identified, RNA molecules must be bound to ribosomes, and the presence of introns must be considered. Collecting the gene product and exporting it from the cell are other considerations. The genes used in DNA technology are commonly obtained from host cells or organisms called gene libraries . A gene library is a collection of cells identified as harboring a specific gene. For example, E. coli cells can be stored with the genes for human insulin in their chromosomes....
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