Diagnostic Testing

Diagnostic Testing - Diagnostic Testing Recombinant DNA and...

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Unformatted text preview: Diagnostic Testing Recombinant DNA and biotechnology have opened a new era of diagnostic testing and have made detecting many genetic diseases possible. The basic tool of DNA analyses is a fragment of DNA called the DNA probe . A DNA probe is a relatively small, single-stranded fragment of DNA that recognizes and binds to a complementary section of DNA on a larger DNA molecule. The probe mingles within the mixture of DNA molecules and unites with the target DNA much like a left hand unites with a right. After the probe unites with its target, it emits a signal like that from a radioactive isotope to indicate that a reaction has occurred. To work effectively, a sufficiently large amount of target DNA must be available. To increase the amount of available DNA, a process called the polymerase chain reaction (PCR) is used. In a highly automated machine, the target DNA is combined with enzymes, nucleotides, and a primer...
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This note was uploaded on 11/11/2011 for the course BIO 101 taught by Professor Pesthy during the Fall '07 term at Texas State.

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