Viral Cultivation and Physiology

Viral Cultivation and Physiology - then inoculated with...

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Viral Cultivation and Physiology Viruses can be cultivated within suitable hosts, such as a living cell. To study  bacteriophages, for example, bacteria are grown in a suitable growth medium; then  bacteriophages are added. The bacteriophages multiply within the bacteria and increase  their numbers substantially. Animal and plant viruses are cultivated in cell cultures. A  cell culture  is prepared by  encouraging cell growth outside the animal or plant source. The cells are kept alive in a  suspension of growth factors within a Petri dish. A thin layer of cells, or monolayer, is 
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Unformatted text preview: then inoculated with viruses, and replication takes place. Fertilized eggs and living animals can also be used to cultivate viruses. For research study, viruses can be cultivated in large volumes by inoculations to tissue culture systems. After a time, the cells are degenerated, and viruses are harvested. The viral particles are concentrated by precipitation methods and purified by repeated centrifugations. Highly purified viruses can be obtained by crystallization and concentration under established conditions....
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This note was uploaded on 11/11/2011 for the course BIO 101 taught by Professor Pesthy during the Fall '07 term at Texas State.

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