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exam2_key - 20.180 Exam 2 11 May 2006 Closed Book Name _...

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20.180 Exam 2 Name ___________________ 11 May 2006 Closed Book Question 1 (35 points total) Refer to the standard genetic code table if needed. A. Circle any open reading frame(s) encoded in the double stranded DNA fragment shown below (10 points). 5’-TTA ATGGTGCCGCGG CATTAA -3’ 3’- AATTACCACGGCGCC GTA ATT-5’ The restriction endonuclease SacII cuts the DNA sequence shown here: 5’-CCGCGG-3’ 3’-GGCGCC-5’ B. Underline any SacII site(s) on the DNA shown in part A (5 points). C. Design a new piece of DNA that does not contain any SacII site(s) but that still encodes the same protein(s) as the DNA shown in part A (15 points). Before DNA: TTA ATGGTGCCGCGG CATTAA AATTACCACGGCGCC GTA ATT ORF1: ATG GTG CCG CGG CAT TAA Met Val Pro Arg His * ORF2: ATG CCG CGG CAC CAT TAA Met Pro Arg His His * After DNA : TTA ATGGTGCCtCGG CATTAA AATTACCACGGaGCC GTA ATT ORF1: ATG GTG CCt CGG CAT TAA Met Val Pro Arg His * ORF2: ATG CCG aGG CAC CAT TAA Met Pro Arg His His *
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20.180 Exam 2 Name ___________________
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This note was uploaded on 11/11/2011 for the course BIO 20.010j taught by Professor Lindagriffith during the Spring '06 term at MIT.

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exam2_key - 20.180 Exam 2 11 May 2006 Closed Book Name _...

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